Comparison of different pretreatments in ethanol fermentation using corn cob hemicellulosic hydrolysate with Pichia stipitis and Candida shehatae

摘要

A hemicellulosic hydrolysate was prepared with 0.3 M H_2SO_4 at 98deg C for 1 h. The total initial reducing sugar was maintained at 45 g l~(-1) by synthetic xylose supplementation. The seven detoxification methods were employed including either the single addition of solid CaO (to pH 10 or 6) or its combinations with zeolite shaking. Over-liming gave the hydrolysate that was most completely fermented by Pichia stipitis and Candida shehatae at 30deg C, pH 6, among the tested methods.