Possibility of classification in some species of Lilium by PCR-RFLP of ribulose-1,5-bisphosphate carboxylase large subunit (rbcL) gene and ribosomal RNA gene.

摘要

PCR-RFLP analysis was applied to classify 9 Lilium species (L. japonicum, L. rubellum, L. nobilissimum, L. speciosum, L. auratum, L. auratum var. platyphyllum, L. henryi, L. concolor var. pulchellum and L. leichtlinii var. maximowiczii) and one interspecific hybrid, L. X formolongi. When PCR products amplified in specific regions of ribulose-1,5-bisphosphate carboxylase large subunit gene (rbcL) and nuclear ribosomal RNA (rRNA) gene were digested by 20 restriction enzymes, some polymorphisms were observed. The examined plants were classified into 6 groups based on the combined results from these genes. The tested species in Archelirion and Sinomartagon were classified into different groups. Furthermore, 6 species in Archelirion were classified into 3 groups. Thus, this study indicates that PCR-RFLP analysis could be used for classification of lilies.