Randomly amplified polymorphic DNA analysis for establishing phylogenetic relationship among Cattleya walkeriana Gardn., Cattleya nobilior Rchb. f. and Cattleya loddigesii Lindl.

摘要

Twenty-five cultivars of C. walkeriana, including one with an obscure original source and phylogenetically unidentified, C. nobilior, C. loddigesii, and their hybrids, were examined by RAPD analysis in an attempt establish their phylogenetic relationships and to distinguish cultivars with different flower colours. Eight 12- and 10-mer primers generated 176 polymorphic bands between 100 and 2000 bp, out of which 65 bands were used for cluster analysis. Three distinct clusters representing C. walkeriana, C. nobilior and C. loddigesii were obtained, indicating that C. nobilior was clearly a different species from C. walkeriana in accordance with the classification of Brieger et al. (1981). C. walkeriana var. alba 'Pendentive' is a presumed cultivar of C. nobilior or a hybrid of C. loddigesii, but fell into the same cluster as other cultivars of C. walkeriana var. alba. C. walkeriana cultivars with different flower colours were distributed into different clusters by RAPD analysis when only one primer was used.