Monitoring of specific methanogenic activity of granular suldge by confocal laser scanning microscopy during start-up of thermophilic upflow anaerobic sludge blanket reactor

摘要

Confocal,laser-scanning microscopy was applied to acquire coenzyme F_(420)-based autofluorescence images of middle sections of sludge granules during start-up of a thermophilic reactor that were seeded with mesophilicallygrown microorganisms of granular sludge.Digital images were analyzed to calculate weighted averages of autofluorescence.The values were related (r~2=0.97) to specific methanogenic activities of granular sludge as the granules developed to steady state.