Profiling of StvacINV1, BAM1 and INH2 alpha Expressions in Relation to Acid Invertase and beta-Amylase Activities During Development of Cold-Induced Sweetening in Indian Potato (Solanum tuberosum L.) Tubers

摘要

Cold-induced sweetening (CIS) characterized by reducing sugars (RS) accumulation during low temperature storage of potato (Solanum tuberosum L.) tubers remains a serious postharvest concern for the potato processing industry. Enzymes involved in carbohydrates metabolism and the genes modulating their activities are of paramount importance in the events associated with the development of CIS. Expression of vacuolar acid invertase gene StvacINV1, beta-amylase gene BAM1 and invertase inhibitor gene INH2 alpha and their consequence on acid invertase and beta-amylase activities with resulting RS accumulation were followed in one CIS-tolerant (Kufri Jyoti) and one CIS-susceptible (Kufri Badshah) Indian potato varieties stored in cold conditions. Differential gene expression analysis showed that during cold storage, expression of StvacINV1 and BAM1 increased at low temperature and their transcripts were more expressed in the CIS-tolerant variety than the CIS-sensitive. Besides, correlation between BAM1 expression and beta-amylase activity affirmed the hypothesis of several enzymes and pathways involved in starch degradation during cold storage of potato. Expression of invertase inhibitor gene INH2 alpha however was higher in the CIS-tolerant variety than the CIS-sensitive. Correlating StvacINV1 and INH2 alpha expressions with RS content and acid invertase activity established that post-translational regulation of acid invertase by the invertase inhibitor protein could be an important component of resistance to CIS.