Detection of ALK and KRAS Mutations in Circulating Tumor DNA of Patients With Advanced ALK-Positive NSCLC With Disease Progression During Crizotinib Treatment

摘要

We investigated mechanisms of acquired resistance in a group of anaplastic lymphoma kinase (ALK) translocated lung cancer patients whose disease progressed during crizotinib treatment using plasmatic circulating DNA extracted from blood samples. We identified 5 patients who presented ALK plasmatic mutations and 10 patients with Kirsten rat sarcoma (KRAS) gene mutations, both associated with crizotinib resistance. We showed that plasmatic decrease of mutation levels was associated with radiological response confirming that resistance ALK and KRAS mutations are detectable and their monitoring could serve as response parameters.