Microsatellite loci in Ipomopsis aggregata (Polemoniaceae) and cross-species applicability for ecological genetics studies.

摘要

Premise of the study: Novel microsatellite primers were developed for the native wildflower Ipomopsis aggregata to facilitate ongoing studies of the genetics of local adaptation and patterns of hybridization with closely related species within the genus. Methods and Results: Thirteen primer sets were successfully developed and tested using populations from the Rocky Mountains of Colorado, USA. The primers amplified di-, tri-, and tetranucleotide repeats with 1-15 alleles per locus. All primers also amplified fragments with varying success in closely related Ipomopsis species and more distant members of the Polemoniaceae. Conclusions: The polymorphism levels observed across all loci suggest that these microsatellites may be useful for population genetic studies in Ipomopsis, as well as in studies of other related taxa in the Polemoniaceae.Digital Object Identifier http://dx.doi.org/10.3732/ajb.1100612