Antioxidant Activity and Neuroprotective Effect of Root Bark of Morns alba L. Extract against Hydrogen Peroxide-Induced Cytotoxicity in PC 12 Cells

摘要

In this study, the antioxidant activity and neuroprotective effect of root bark of Moms alba L. on H_2O_2-induced differentiated PC 12 cells were investigated. In order that, total phenolic and flavonoid contents of Morns alba L. extract in differentethanol concentrations (0, 30, 50, 70, and 100%) were measured. Antioxidant activities has been evaluated by oxygen radical absorbance capacity assay, l,l-diphenyl-2-picrylhydrazyl radical scavenging assay, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay, ferric reducing antioxidant power assay, and Fe2+ chelate assay. The results suggested that 70% ethanol extract of root bark of Morus alba L. had high phenolic and flavonoid contents (35.0±1.5 mg/g, 7.9±0.2 mg/g) and antioxidant activities. The 70% ethanol extract of root bark of Morus alba L., which showed the most antioxidative activities, was treated on ITCVinduced differentiated PC 12 cells in order to evaluate its cell protective effect by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell membrane protective effect by lactate dehydrogenase (LDH) assay. The results showed that cell viability was increased when treated with 25 — 200 ug/mL for 70% ethanol extract of root bark of Morus alba L., and LDH amount was decreased in a dose dependant manner. The results of this study demonstrate that Morus alba L. has potent antioxidant and neuroprotective effects against H_2O_2-induced oxidative stress.