Optimization of beta-1,3/1,6-Glucan Production of Aureobasidium pullulans KSY-0516 Strain

摘要

beta-Glucans are major bioactive compounds known to have biological activities, including anti-cancer, anti-inflammatory, and immune-modulating properties. Aureobasidium pullulans, known as black yeast, produces a variety of black p-glucans, which canbe used to produce white p-1,3/1,6-glucan using a mutation technique. The purpose of this study was to determine the optimum production yield of p-1,3/1,6-glucan using response surface methodology, which is a statistical procedure used for optimizationstudies. A central composite design was applied to investigate the effects of independent variables, sucrose concentration (X_1), KNO_3 concentration (X_2), and oxalic acid concentration (X_3) on responses such as yield and P-1,3/1,6-glucan production rate. A maximum yield of 1.86217 g/L was obtained at 4.74% sucrose, 0.19% KNO3, and 0.036% oxalic acid. Based on the above results, the optimal production and efficient production of P-glucans can be achieved.