Inhibitory Effect of Cell Differentiation against 3T3-L1 Pre-Adipocytes and Angiotensin Converting Enzyme (ACE) Activity of Ice Plant {Mesembryanthemum crystallinum)

摘要

Mesembryanthemum crystallinum (Family: Aizoaceae) is an annual plant consisting of ice crystal-shaped bladder cells, which is responsible for its common name ice plant. This study investigated biological activities according to general components andextraction solvent in order to examine the functionality of ice plant. The total content of free amino acids was 32.57 mg/g, including 4.64 mg/g of L-alanine as the most abundant and 2.60 mg/g of y-amino-butyric acid. Regarding angiotensin converting enzyme inhibitory activities of solvent fractions of ice plant, ethyl acetate fraction and chloroform fraction showed activities of 33.17±3.20~88.19±3.20% and 23.72±2.89~86.78±2.24%, respectively, similar to Captopril? (19.51 ±3.44~84.72± 1.06%) and Enalapril? (24.93±1.12~91.32±3.62%) as positive control groups. Regarding inhibition of lipid droplet production in 3T3-L1 preadipocytes by ice plant, anti-adipogenic activities were 53.00±0.45~65.75±0.31% and 44.16±0.29~63.32±0.36% in the ethyl acetate fraction and butanol fraction, respectively, showing the lowest lipid droplet production. The chloroform fraction and hexane fraction showed activities of 38.33±0.09~56.55±0.50% and 31.17±0.50 ~ 55.10± 1.93%, respectively, whereas the water fraction showed activity of 26.32±2.27~49.48±0.05%. Therefore, all solvent fractions inhibited fat accumulation of 3T3-L1 preadipocytes according to treatment concentration. According to the results above, it would be possible to utilize ice plant as a newhealth functional material.