Effects of supplementary vitamin C and E to moist pellet diet on growth and body composition of juvenile olive flounder, Paralichthys olivaceus

摘要

This study was conducted to investigate the supplementary effects of vitamin C and E to moist pellet on growth and body composition in juvenile olive flounder (Paralichthys olivaceus). Four groups of diet made : moist pellet (MP) diet group was usedas the control group where the ratio of raw feed and commercial compound meal is 5:5 (CP 30 percent, CL 17 percent), vitamin C added group (VC), vitamin E added group (VE) and vitamin C and E added group (VCE). The supplementary amount of vitamin C andE corresponded to 1000 mg/kg (dry wt.) and 220 mg/kg (dry wt.), respectively. Vitamin C was destroyed 50 percent and vitamin E was destroyed 20 percent for manufacturing process. After 8 weeks feeding trial, weight gain was 121.9 percent in MP group, while it ranged from 180.5 to 184.9 percent in the VC. VE and VCE group. Feed efficiency was 71.6 percent in MP group, whereas it ranged from 78.7 to 80.6 percent in the VC, VE and VCE group, weight gain and feed efficiency of fish fed vitamin supplementeddiets were significantly higher than control group and no significant difference among the vitamin added groups was observed (p>0.05). There were no significant differences in moisture, crude protein, crude Upid, crude ash. condition factor (CF). hepatosomatic index (HSI), and visceraweight index (VWI) of body composition. Hemoglobin (Hb) was 3.79 percent in MP group, whereas 4.99 percent in VC group, 4.55 percent in VE group and 5.07 percent in VCE group; Hb was significantly higher in the vitamin added groups than (he control group (p<0.05). Vitamin C retention in liver was 18.1, 20.7, 46.6, 54.0 mg/kg in MP. VE. VC and VCE groups, respectively. Vitamin E retention in liver was 25.9, 53.8, 88.2, 124.5 mg/kg in MP, VC, VE and VCE groups, respectively.These results indicate that supplementation of vitamin C and E to moist pellet diet significantly improved growth and feed efficiency of olive flounder, and the mixed addition of vitamin C (1,000 mg/kg dry wt.) and vitamin E (220 mg/kg dry wt.) did notlead to the synergy effect.