Optimization and Characterization of Biosurfactant Rhamnolipid Production by Pseudomonas aeruginosa Isolated from an Artificially Contaminated Soil

Camara Jéssica Maria D.A.; Sousa Magna Angélica S.B.; Barros Neto Eduardo L.

摘要

Abstract > Biosurfactants are surfactants biologically produced by microorganisms, presenting several advantages when compared to synthetic surfactants. <fc> <fi>Pseudomonas aeruginosa</fi> </fc> is known for producing rhamnolipids, considered one of the most interesting types of biosurfactants due to their high yields, when compared to other types. In this work, the production of rhamnolipid from <fc> <fi>P. aeruginosa</fi> </fc> was optimized. At first, the Plackett–Burman design was used to select most significant variables affecting the biosurfactant production yield among nine variables—carbon–nitrogen ratio, carbon concentration, nitrogen source, pH, cultivation time, potassium and magnesium concentrations, agitation, and temperature. Then, using main variables, a central point experimental design aiming to optimize rhamnolipid production was performed. The maximum biosurfactant concentration obtained was 0.877 mg L ?1 . The rhamnolipid also displayed a great emulsification rate, reaching approximately 67%, and the ability to reduce water surface tension from 72.02 to 35.26?mN?m ?1 at a critical micelle concentration (CMC) of 127 mg L ?1 , in addition to presenting a good stability when exposed to wide pH and salinity ranges. The results suggest that rhamnolipids are promising substitutes for synthetic surfactants, especially due to lower impacts on the environment. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 生物表面活性剂是通过微生物制造的表面活性剂，与合成表面活性剂相比，呈现出几种优点。 <FC> <fi>假单胞菌铜绿假单胞菌</ fi> </ FC> 众所周知，与其他类型相比，由于其高产率而认为，被认为是最有趣的生物活性剂类型之一。在这项工作中，生产rhamnolipid的 <FC> <fi> p。 Aruginosa </ Fi> </ FC> 优化了。起初，Plackett-Burman设计用于选择影响九个变量 - 碳氮比，碳浓度，氮源，pH，培养时间，钾和镁浓度，搅拌和温度的最重要变量。然后，使用主要变量，进行旨在优化耳鼻疙瘩产生的中心点实验设计。获得的最大生物活性剂浓度为0.877mg ？1 </ sup> 。 rhamnolipid也显示出很大的乳化率，达到大约67％，以及将水面张力从72.02降低到35.26的能力。Mn？m ？1 </ sup> 在临界胶束浓度（CMC）为127 mg l ？1 </ sup> 除了在暴露于宽pH和盐度范围的情况下呈现良好的稳定性。结果表明，rhamnolipids是合成表面活性剂的有前途的替代品，特别是由于对环境的较低影响。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35863/'>《Journal of Surfactants and Detergents》</a> <b style="margin: 0 2px;">|</b><span>2019年第4期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Camara Jéssica Maria D.A.&option=202" target="_blank" rel="nofollow">Camara Jéssica Maria D.A.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sousa Magna Angélica S.B.&option=202" target="_blank" rel="nofollow">Sousa Magna Angélica S.B.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Barros Neto Eduardo L.&option=202" target="_blank" rel="nofollow">Barros Neto Eduardo L.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Chemical Engineering DepartmentUniversidade Federal do Rio Grande do NorteSenador Salgado Filho 3000—Campus Universitário Natal Rio Grande do Norte Brazil;</p> <p>Chemical Engineering DepartmentUniversidade Federal do Rio Grande do NorteSenador Salgado Filho 3000—Campus Universitário Natal Rio Grande do Norte Brazil;</p> <p>Chemical Engineering DepartmentUniversidade Federal do Rio Grande do NorteSenador Salgado Filho 3000—Campus Universitário Natal Rio Grande do Norte Brazil;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4818.html" title="表面现象的物理化学">表面现象的物理化学;</a><a href="https://www.zhangqiaokeyan.com/clc/7878.html" title="表面活性剂">表面活性剂;</a><a href="https://www.zhangqiaokeyan.com/clc/1844.html" title="油脂和蜡的化学加工工业、肥皂工业">油脂和蜡的化学加工工业、肥皂工业;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rhamnolipid&option=203" rel="nofollow">Rhamnolipid;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pseudomonas aeruginosa&option=203" rel="nofollow">Pseudomonas aeruginosa;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Optimization&option=203" rel="nofollow">Optimization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Biosurfactants&option=203" rel="nofollow">Biosurfactants;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Experimental design&option=203" rel="nofollow">Experimental design;</a> </p> <div class="translation"> 机译：rhamnolipid;假单胞菌铜绿假单胞菌;优化;生物表面活性剂;实验设计; </div> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul id="commoncitationul"></ul> <ul id="tworeferenceul"></ul> <ul id="twocitationul"></ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025864222.html">Optimization and Characterization of Biosurfactant Rhamnolipid Production by Pseudomonas aeruginosa Isolated from an Artificially Contaminated Soil</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Camara Jéssica Maria D.A.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Camara Jéssica Maria D.A.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sousa Magna Angélica S.B.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sousa Magna Angélica S.B.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Barros Neto Eduardo L.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Barros Neto Eduardo L. </a> <a href="/journal-foreign-35863/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Surfactants and Detergents .</a> <span>2019</span><span>,第4期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：铜绿假单胞菌分离出人工污染土壤的生物活性剂犀牛产量的优化与表征</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070404998912.html">Biosurfactant production by Pseudomonas aeruginosa DSVP20 isolated from petroleum hydrocarbon-contaminated soil and its physicochemical characterization</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sharma Deepak&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sharma Deepak,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ansari Mohammad Javed&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ansari Mohammad Javed,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Al-Ghamdi Ahmad&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Al-Ghamdi Ahmad,</a> <a href="/journal-foreign-22738/" target="_blank" rel="nofollow" class="tuijian_authcolor">Environmental Science and Pollution Research .</a> <span>2015</span><span>,第22期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：铜绿假单胞菌DSVP20从石油烃污染土壤中分离出来的生物表面活性剂的生产及其理化特性</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070407988629.html">Characterization of glycolipid biosurfactant from Pseudomonas aeruginosa CPCL isolated from petroleum-contaminated soil</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Arutchelvi J.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Arutchelvi J.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Doble M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Doble M. </a> <a href="/journal-foreign-26428/" target="_blank" rel="nofollow" class="tuijian_authcolor">Letters in Applied Microbiology .</a> <span>2010</span><span>,第1期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：石油污染土壤中铜绿假单胞菌CPCL中糖脂生物表面活性剂的表征</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-194173_thesis/020513133536.html">Enhancement of Rhamnolipid Production in Residual Soybean Oil by an Isolated Strain of Pseudomonas aeruginosa</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=C. 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Jayachandran </a> <span>2013</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用新型铜绿假单胞菌渣油油炸椰子油从废物煎炸椰子油的生产和表征</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_acta-pedologica-sinica_thesis/0201257140006.html">生物表面活性剂强化微生物修复多环芳烃污染土壤的初探</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘魏魏&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘魏魏</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=尹睿&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,尹睿</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=林先贵&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,林先贵</a> <span> <a href="/journal-cn-6780/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 土壤学报 </a> </span> <span> . 2010</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-anhui-agricultural-sciences_thesis/020122315203.html">生物表面活性剂在石油污染土壤生物修复中的应用</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=乔德波&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 乔德波</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=牛明芬&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,牛明芬</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐丽&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,徐丽</a> <span> <a href="/journal-cn-7281/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 安徽农业科学 </a> </span> <span> . 2007</span><span>,第028期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_geology-in-china_thesis/0201252104595.html">济南市土壤重金属污染现状及其土壤生物学表征</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王存龙&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王存龙</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=董志成&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,董志成</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=夏学齐&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,夏学齐</a> <span> <a href="/journal-cn-4800/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国地质 </a> </span> <span> . 2012</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-zhejiang-university-agriculture-life-sciences_thesis/02012961391.html">铜绿假单胞菌M2联合小白菜对Pb污染土壤的生物修复</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈宝&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈宝</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐晓萌&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,徐晓萌</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曲娟娟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,曲娟娟</a> <span> <a href="/journal-cn-7268/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 浙江大学学报（农业与生命科学版） </a> </span> <span> . 2012</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201296058247.html">表面活性剂对人工污染土壤装填土柱中PAHs迁移渗透的影响</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈静&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈静</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王学军&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王学军</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=胡俊栋&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,胡俊栋</a> <span> <a href="/journal-cn-56690/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 环境科学 </a> </span> <span> . 2005</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-3861_thesis/02022893785.html">利用产生物表面活性剂菌株土柱淋洗修复重金属污染土壤</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Zhihui&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Yang Zhihui</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨志辉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨志辉</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Zhi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Zhang Zhi</a> <span> <a href="/conference-cn-3861/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国有色金属冶金第一届学术会议 </a> <span> <span> . 2014</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020313204365.html">生物表面活性剂强化的石油污染土壤生物修复研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=冯冬梅&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 冯冬梅</a> <span> . 2019</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061203152866.html">一种生物表面活性剂强化植物—微生物共生体系的方法及其在原位修复铀污染土壤中的应用</a> <b>[P]</b> . <span> 中国专利： CN105689375B </span> <span> . 2019.01.18</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120100068825.html">生物炭负载表面活性剂耦合微生物植物修复石油污染土壤的方法</a> <b>[P]</b> . <span> 中国专利： CN109365495A </span> <span> . 2019-02-22</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130423374089.html">RHAMNOLIPID BIOSURFACTANT FROM PSEUDOMONAS AERUGINOSA STRAIN NY3 AND METHODS OF USE</a> <b>[P]</b> . <span> 外国专利：  US2011306569A1 </span> <span> . 2011-12-15</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：铜绿假单胞菌菌株NY3鼠李糖脂生物表面活性剂及其使用方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130405870244.html">Pseudomonas aeruginosa TSKW-S5 Pseudomonas aeruginosa TSKW-S5 strain having oil resolution and oil-releasing ability a method for purifying contaminated soil using the same and a method for selecting the strain</a> <b>[P]</b> . <span> 外国专利：  KR101896428B1 </span> <span> . 2018-09-10</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：具有油分离和释油能力的铜绿假单胞菌TSKW-S5菌株，使用该菌株纯化污染土壤的方法和选择菌株的方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130439389292.html">THE MARINE BACTERIUM PSEUDOMONAS AERUGINOSA BYK-2 PRODUCING RHAMNOLIPID AND METHYL RHAMNOLIPID SIMULTANEOUSLY</a> <b>[P]</b> . <span> 外国专利：  KR100458136B1 </span> <span> . 2004-11-26</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：同时生产鼠李糖脂和甲基鼠李糖脂的海洋细菌铜绿假单胞菌BYK-2 </span> </p> </li> </ul> 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