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首页> 外文期刊>Journal of separation science. >Capillary electrophoresis with capacitively coupled contactless conductivity detection method development and validation for the determination of azithromycin, clarithromycin, and clindamycin
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Capillary electrophoresis with capacitively coupled contactless conductivity detection method development and validation for the determination of azithromycin, clarithromycin, and clindamycin

机译：具有电容耦合非接触式电导检测方法的毛细管电泳，测定阿奇霉素，克拉霉素和克林霉素的测定

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Abstract > A capillary electrophoresis with capacitively coupled contactless conductivity detection based method for the assay of azithromycin, clarithromycin and clindamycin was optimized and validated in this study. A buffer solution of 20?mM 2‐( N ‐morpholino) ethane sulfonic acid, 40?mM <sc>l</sc> ‐histidine and 0.6?mM cetyltrimethylammonium bromide (pH 6.39) was used for the electrophoresis. An uncoated, bare‐fused silica capillary (total length 60?cm, effective length 32?cm, 75?μm id) was used at 25°C. The sample was injected hydrodynamically at 0.5?psi for 5?s. The electrophoresis was conducted at 30?kV in reverse polarity for 6?min with 3 and 2?min of in‐between sodium hydroxide (0.1?M) and background electrolyte rinsing, respectively. Ammonium acetate was used as internal standard. This simple and robust method showed reasonable limit of detection and limit of quantitation for azithromycin (0.0125/0.03?mg/mL), clarithromycin (0.017/0.03?mg/mL), and clindamycin (0.038/0.06?mg/mL), with good selectivity, precision both intraday (relative standard deviation?≤?1.0%) and interday (relative standard deviation??3.7%), linearity ( R 2 ??0.999) and recovery (99?–?101.7%). The method was successfully applied for the determination of azithromycin, clarithromycin and clindamycin in formulations. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”> <标题类型=“main”>摘要</标题> >毛细管电泳，具有电容耦合的非接触式电导检测的基于阿奇霉素，克朗霉素和Clindamycin的测定方法进行了优化和验证学习。使用缓冲溶液，20μm2-（ n℃-morpholino）乙烷磺酸，40×mm温度> -histidine和0.6？mm十六烷基三甲基溴铵（pH6.39）用于电泳。在25℃下使用未涂覆的裸熔融二氧化硅毛细管（总长度60Ωcm，有效长度32Ωcm，75μmid）。将样品在0.5℃下注入0.5℃的5℃。电泳，在30×kV的反向极性下进行6〜min，分别在氢氧化钠（0.1Ωm）和背景电解质漂洗中3和2·min。乙酸铵用作内标。这种简单且稳健的方法显示出氮霉素（0.0125 /0.03Ωmg/ ml），克林霉素（0.017 /0.03Ω·mg / ml）和Clindamycin（0.038 /0.06Ω·mg / ml）的合理限制。良好的选择性，精确地（相对标准偏差？≤≤1.0％）和内部（相对标准偏差？<3.7％），线性度（ R </ i> 2 </ sup>？＆gt ; 0.999）和恢复（99？ - ？101.7％）。该方法已成功地应用于亚辛霉素，克拉霉素和克林霉素中的制剂中的含量。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2017年第17期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Paul Prasanta&option=202" target="_blank" rel="nofollow">Paul Prasanta;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Duchateau Tom&option=202" target="_blank" rel="nofollow">Duchateau Tom;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=S?nger‐van de Griend Cari&option=202" target="_blank" rel="nofollow">S?nger‐van de Griend Cari;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Adams Erwin&option=202" target="_blank" rel="nofollow">Adams Erwin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Schepdael Ann&option=202" target="_blank" rel="nofollow">Schepdael Ann;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>KU Leuven ‐ University of LeuvenDepartment of Pharmaceutical and Pharmacological SciencesLeuven Belgium;</p> <p>KU Leuven ‐ University of LeuvenDepartment of Pharmaceutical and Pharmacological SciencesLeuven Belgium;</p> <p>Analytical Pharmaceutical ChemistryUppsala UniversityUppsala Sweden;</p> <p>KU Leuven ‐ University of LeuvenDepartment of Pharmaceutical and Pharmacological SciencesLeuven Belgium;</p> <p>KU Leuven ‐ University of LeuvenDepartment of Pharmaceutical and Pharmacological SciencesLeuven Belgium;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=azithromycin&option=203" rel="nofollow">azithromycin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=capacitively coupled contactless conductivity detection&option=203" rel="nofollow">capacitively coupled contactless conductivity detection;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=capillary electrophoresis&option=203" rel="nofollow">capillary electrophoresis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=clarithromycin&option=203" rel="nofollow">clarithromycin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=clindamycin&option=203" rel="nofollow">clindamycin;</a> </p> <div class="translation"> 机译：氮杂霉素;电容耦合非接触式电导检测;毛细管电泳;克朗霉素;Clindamycin; 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈朋飞&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈朋飞</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=吴成新&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,吴成新</a> <span> <a href="/conference-cn-41419/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第21届全国色谱学术报告会 </a> <span> <span> . -1</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315600438.html">毛细管电泳-电容耦合非接触式电导检测体系的构建及其在食品分析中的应用</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=权菲菲&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 权菲菲</a> <span> . 2018</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061201572954.html">注射用盐酸克林霉素杂质的检测方法及含量测定方法</a> <b>[P]</b> . <span> 中国专利： CN103487518B </span> <span> . 2015.03.18</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120102213748.html">注射用盐酸克林霉素杂质的检测方法及含量测定方法</a> <b>[P]</b> . <span> 中国专利： CN103487518A </span> <span> . 2014-01-01</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130439022422.html">Measurement unit, for capillary electrophoresis, has a capillary array in a micro-fluid chip within a box with conductive walls, a high tension supply and a high frequency transmitter/receiver for non-contact conductivity detection</a> <b>[P]</b> . <span> 外国专利： <!-- 德国专利： --> DE202005009960U1 </span> <span> . 2005-09-01</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于毛细管电泳的测量单元在带导电壁的盒子内的微流体芯片中具有毛细管阵列，高压电源和用于非接触式电导率检测的高频发射器/接收器 </span> </p> </li> <li> <div> <b>4. </b><a 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