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首页> 外文期刊>Journal of separation science. >Efficient separation of high‐purity compounds from Oxytropis falcataOxytropis falcata using two‐dimensional preparative chromatography
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Efficient separation of high‐purity compounds from Oxytropis falcataOxytropis falcata using two‐dimensional preparative chromatography

机译：利用二维制备色谱法从氧暴含量的氧暴含量的氧暴

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Abstract > The separation of high‐purity compounds from traditional Tibetan medicines plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate compounds with high purity because of the complexity of traditional Tibetan medicines. In this work, an offline two‐dimensional reversed‐phase preparative method was successfully developed for the separation of high‐purity compounds from Oxytropis falcata . Based on the analysis results, an ODS C18 prep column was used for first‐dimensional preparation, and 14.8?g of the crude sample was separated into five fractions with a recovery of 74.6%. Then, an XAqua C18 prep column was used to isolate high‐purity compounds in the second‐dimensional preparation because its separation selectivity is different with the ODS C18 stationary phase. As a result, eight compounds in the crude sample were isolated in more than 98% purity. This is the first report of trans ‐cinnamic acid ( 1 ) and trifolirhizin ( 2 ) from Oxytropis falcata . This method has the potential to be an efficient separation method of high‐purity compounds from Oxytropis falcata and it shows great promise for the separation of high‐purity compounds from complex samples. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”> <标题类型=“main”>摘要</ title> >来自传统西藏药物的高纯度化合物的分离在调查其生物活性方面发挥着重要作用。然而，由于传统藏药物的复杂性，常常难以使纯度高纯度的化合物。在这项工作中，成功开发了离线二维反转相制备方法，用于从 oxicropis falcata </ i>分离高纯度化合物。基于分析结果，使用ODS C18预备柱进行一维制剂，将14.8μl粗样品分离成五个级分，回收率为74.6％。然后，使用XAQUA C18准备柱用于在第二维制剂中分离高纯度化合物，因为其分离选择性与ODS C18固定相不同。结果，将粗产物中的8种化合物分离出超过98％的纯度。这是来自 oxcoxtropis falcata </ i>的第一个innamic acid（ 1 / b>）和三升（ 2 / b>）的第一个报告。该方法具有来自 oxicropis falcata的高纯度化合物的有效分离方法</ i>，它表明了从复杂样品中分离高纯度化合物的许多希望。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2017年第18期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Weidong&option=202" target="_blank" rel="nofollow">Wang Weidong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tao Yanduo&option=202" target="_blank" rel="nofollow">Tao Yanduo;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jiao Lijin&option=202" target="_blank" rel="nofollow">Jiao Lijin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fan Minxia&option=202" target="_blank" rel="nofollow">Fan Minxia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shao Yun&option=202" target="_blank" rel="nofollow">Shao Yun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Qilan&option=202" target="_blank" rel="nofollow">Wang Qilan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mei Lijuan&option=202" target="_blank" rel="nofollow">Mei Lijuan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dang Jun&option=202" target="_blank" rel="nofollow">Dang Jun;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau BiologyChinese Academy of SciencesXining Qinghai China;</p> <p>Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau BiologyChinese Academy of SciencesXining Qinghai China;</p> <p>Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau BiologyChinese Academy of SciencesXining Qinghai China;</p> <p>Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau BiologyChinese Academy of SciencesXining Qinghai China;</p> <p>Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau BiologyChinese Academy of SciencesXining Qinghai China;</p> <p>Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau BiologyChinese Academy of SciencesXining Qinghai China;</p> <p>Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau BiologyChinese Academy of SciencesXining Qinghai China;</p> <p>Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau BiologyChinese Academy of SciencesXining Qinghai China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=high‐purity compounds&option=203" rel="nofollow">high‐purity compounds;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Oxytropis falcata&option=203" rel="nofollow">Oxytropis falcata;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=traditional Tibetan medicines&option=203" rel="nofollow">traditional Tibetan medicines;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=two‐dimensional liquid chromatography&option=203" rel="nofollow">two‐dimensional liquid chromatography;</a> </p> <div class="translation"> 机译：高纯度化合物;oxytropis falcata;传统的西藏药物;二维液相色谱; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025846404.html">Efficient separation of high‐purity compounds from <i >Oxytropis falcataOxytropis falcata using two‐dimensional preparative chromatography</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Weidong&option=202" 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曹雪莹&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 曹雪莹</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=冯卫&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,冯卫</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄寿辉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,黄寿辉</a> <span> <a href="/journal-cn-9125/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 广州化工 </a> </span> <span> . 2021</span><span>,第010期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-1572_thesis/02022217986.html">高效液相色谱法测定暴贝止咳口服液中紫丁香苷的含量</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄炳昌&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 黄炳昌</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=殷金龙&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,殷金龙</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐德辉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,徐德辉</a> <span> <a href="/conference-cn-1572/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2015年全国医药学术交流会 </a> <span> <span> . 2015</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/02031191046.html">暴氧污水稀释聚合物驱油试验研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=孙春红&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 孙春红</a> <span> . 2007</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120110521279.html">一种有氧发酵暴气系统及暴气口组件</a> <b>[P]</b> . <span> 中国专利： CN108046863A </span> <span> . 2018-05-18</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120207957337.html">一种用于有氧发酵暴气系统的暴气口组件</a> <b>[P]</b> . <span> 中国专利： CN207958172U </span> <span> . 2018-10-12</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130432832296.html">chromatography binder, methods of preparing a separation matrix, fabricating a chromatography column and separation membrane, and separating one or more antibodies from one or more other compounds in a liquid sample, separation matrix , use thereof, antibody purification kit for one or more other components in a liquid, and disposable chromatography column</a> <b>[P]</b> . <span> 外国专利： <!-- --> BRPI0518216A </span> <span> . 2008-11-04</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：色谱粘合剂，制备分离基质的方法，制造色谱柱和分离膜，以及从液体样品中的一种或多种其他化合物中分离一种或多种抗体的方法，分离基质，其用途，用于一种或多种其他组分的抗体纯化试剂盒在液体和一次性色谱柱中 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130409431871.html">chromatography binder, methods of preparing a separation matrix, and separating one or more antibodies from one or more other compounds into a liquid sample, separation matrix, and disposable chromatography column.</a> <b>[P]</b> . <span> 外国专利： <!-- --> BRPI0518216C8 </span> <span> . 2017-03-21</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：色谱结合剂，分离基质的制备方法以及将一种或多种抗体与一种或多种其他化合物分离成液体样品的方法，分离基质和一次性色谱柱。 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130412231998.html">chromatography binder, methods of preparing a separation matrix, and separating one or more antibodies from one or more other compounds into a liquid sample, separation matrix, and disposable chromatography column.</a> <b>[P]</b> . <span> 外国专利： <!-- --> BRPI0518216B8 </span> <span> . 2016-10-25</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：色谱结合剂，分离基质的制备方法以及将一种或多种抗体与一种或多种其他化合物分离成液体样品的方法，分离基质和一次性色谱柱。 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025846404','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" 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