Characterization of chemical constituents and identification of absorbed components and metabolites in rat plasma of Fu‐Ke‐Zai‐Zao pills by ultra high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry

Ke Han; Tong Wei; Xue Rui; Lu Xiaoyan; Fan Xiaohui

摘要

Abstract >Fu‐Ke‐Zai‐Zao pills, the famous traditional Chinese medicine formula, composed of 42 medicinal herbs, have been widely used to treat various gynecological diseases. However, the chemical constituents and metabolic profiling of Fu‐Ke‐Zai‐Zao pills remain largely unknown, which hampers improvement of the quality control and pharmacological elucidation of this formula. In the present study, a sensitive and selective ultra high performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry method was developed to separate and identify the comprehensive chemical constituents of Fu‐Ke‐Zai‐Zao pills. According to the results, a total of 83 compounds were identified, including phenylpropionic acids, flavonoids, terpenoids, triterpene saponins, and phthalides, and 81 compounds were first reported in Fu‐Ke‐Zai‐Zao pills. Moreover, the absorbed components and metabolites in rat plasma after intragastric administration of Fu‐Ke‐Zai‐Zao pills were also detected by the same analytical method. A total of 36 compounds were identified, including 21 prototypes and 15 metabolites. The latter were generated through the metabolic pathways of methylation and glucuronidation, and glucuronidated metabolites were the main constituents in the plasma. This is the first systematic study on the chemical constituents and metabolic profiling of Fu‐Ke‐Zai‐Zao pills, and the results provide valuable chemical information for further elucidating pharmacological effects and mechanism of action of Fu‐Ke‐Zai‐Zao pills. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >富克Zai Zao丸，着名的中医配方，由42组成药草已被广泛用于治疗各种妇科疾病。然而，富柯枣丸的化学成分和代谢谱重仍然很大程度上是未知的，这妨碍了这种公式的质量控制和药理学阐明的改善。在本研究中，开发了一种与四柱 - 飞行时间质谱法相结合的敏感和选择性超高效液相色谱法，以分离和识别富柯枣丸的综合化学成分。根据结果，鉴定了总共83种化合物，包括苯基丙酸，黄酮类化合物，萜类化合物，三萜素和邻苯二甲酸酯，并在富克曾枣丸中首次报道81种化合物。此外，通过相同的分析方法检测到在富柯瓜毒药肠道尿液施用后大鼠血浆中的吸收成分和代谢物。共鉴定了36种化合物，包括21种原型和15种代谢物。通过甲基化和葡糖醛化的代谢途径产生后者，葡萄糖化代谢物是血浆中的主要成分。这是对富柯枣毒药的化学成分和代谢分析的第一个系统研究，结果提供了有价值的化学信息，以进一步阐明富柯枣丸的药理作用和作用机制。< / p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2019年第10期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ke Han&option=202" target="_blank" rel="nofollow">Ke Han;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tong Wei&option=202" target="_blank" rel="nofollow">Tong Wei;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xue Rui&option=202" target="_blank" rel="nofollow">Xue Rui;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lu Xiaoyan&option=202" target="_blank" rel="nofollow">Lu Xiaoyan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fan Xiaohui&option=202" target="_blank" rel="nofollow">Fan Xiaohui;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou P. R. China;</p> <p>Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou P. R. China;</p> <p>Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou P. R. China;</p> <p>Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou P. R. China;</p> <p>Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=absorbed components&option=203" rel="nofollow">absorbed components;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chemical constituents&option=203" rel="nofollow">chemical constituents;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=mass spectrometry&option=203" rel="nofollow">mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=metabolites&option=203" rel="nofollow">metabolites;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=traditional Chinese medicine&option=203" rel="nofollow">traditional Chinese medicine;</a> </p> <div class="translation"> 机译：吸收成分;化学成分;质谱;代谢物;中医; 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