Identification of metabolites in human and rat urine after oral administration of Xiao‐Qing‐Long‐Tang granule using ultra high performance liquid chromatography combined with quadrupole time‐of‐flight mass spectrometry

Zhou Lei; Zhang Qiang; Qi Wen; Yan Shuai; Qu Jialin; Makino Toshiaki; Yuan Dan

摘要

Abstract > Xiao‐Qing‐Long‐Tang is a traditional Chinese formula used for the treatment of cold syndrome, bronchitis, and nasal allergies for thousands of years. However, the in vivo integrated metabolism of its multiple components and the active chemical constituents of Xiao‐Qing‐Long‐Tang remain unknown. In this study, a method using ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry was established for the detection and identification of the metabolites in human and rat urine after oral administration of Xiao‐Qing‐Long‐Tang. A total of 19 compounds were detected or tentatively identified in human urine samples, including eight prototypes and 11 metabolites. Also, a total of 50 compounds were detected or tentatively identified in rat urine samples, including 15 prototypes and 35 metabolites detected with either a highly sensitive extracted ion chromatogram method or the MS E determination using Mass Fragment software. Our results indicated that phase Ⅱ reactions (e.g. glucuronidation and sulfation) were the main metabolic pathways of flavones, while phase I reactions (e.g. demethylation and hydroxylation) were the major metabolic reaction for alkaloids, lignans, and ginger essential oil. This investigation provided important structural information on the metabolism of Xiao‐Qing‐Long‐Tang and provided evidence to obtain a more comprehensive metabolic profile. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”> <标题类型=“main”>抽象</ title> >萧庆 - 唐唐是一种用于治疗冷综合征，支气管炎和鼻过敏的传统中华公式几千年来。然而，其多种组分的体内综合代谢和小清长汤的活性化学成分仍然未知。在该研究中，建立了使用超高效液相色谱法与四极性飞行时间串联质谱法的方法进行检测和鉴定小清长汤口服施用后人和大鼠尿液中的代谢物。在人尿样中检测或暂时检测到19种化合物，包括八种原型和11种代谢物。此外，在大鼠尿液样品中检测或暂时鉴定了总共50种化合物，其中包含15种原型和35种以高敏感的离子色谱法或MS <I> <SOP> E </ SOP> </ i的原型检测到35种代谢物>使用质量片段软件确定。我们的结果表明，Ⅱ阶段反应（例如葡糖醛酸化和硫酸）是黄酮的主要代谢途径，而I相反应（例如去甲基化和羟基）是生物碱，木质素和姜精油的主要代谢反应。这项调查提供了关于小青唐唐的新陈代谢的重要结构信息，并提供了获得更全面的代谢概况的证据。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2017年第18期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhou Lei&option=202" target="_blank" rel="nofollow">Zhou Lei;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Qiang&option=202" target="_blank" rel="nofollow">Zhang Qiang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Qi Wen&option=202" target="_blank" rel="nofollow">Qi Wen;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yan Shuai&option=202" target="_blank" rel="nofollow">Yan Shuai;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Qu Jialin&option=202" target="_blank" rel="nofollow">Qu Jialin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Makino Toshiaki&option=202" target="_blank" rel="nofollow">Makino Toshiaki;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yuan Dan&option=202" target="_blank" rel="nofollow">Yuan Dan;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Traditional Chinese MedicineShenyang Pharmaceutical UniversityShenyang China;</p> <p>Department of Traditional Chinese MedicineShenyang Pharmaceutical UniversityShenyang China;</p> <p>Department of Traditional Chinese MedicineShenyang Pharmaceutical UniversityShenyang China;</p> <p>Department of Traditional Chinese MedicineShenyang Pharmaceutical UniversityShenyang China;</p> <p>Clinical Laboratory of Integrative Medicine The first affiliated hospital of DalianMedical UniversityDalian China;</p> <p>Department of Pharmacognosy Graduate School of Pharmaceutical SciencesNagoya City UniversityNagoya Japan;</p> <p>Department of Traditional Chinese MedicineShenyang Pharmaceutical UniversityShenyang China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=human urine&option=203" rel="nofollow">human urine;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=mass spectrometry&option=203" rel="nofollow">mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=metabolites&option=203" rel="nofollow">metabolites;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Traditional Chinese medicine&option=203" rel="nofollow">Traditional Chinese medicine;</a> </p> <div class="translation"> 机译：人尿;质谱;代谢物;中医; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=郑育芳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,郑育芳</a> <span> <a href="/journal-cn-14390/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 色谱 </a> </span> <span> . 2006</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_quality-safety-agro-products_thesis/0201279973825.html">超高效液相色谱-四极杆-飞行时间质谱法鉴定羊尿液中安乃近的代谢物</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=安肖&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 安肖</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=毕言锋&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,毕言锋</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐士新&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,徐士新</a> <span> <a href="/journal-cn-6792/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 农产品质量与安全 </a> </span> <span> . 2020</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-forensic-medicine_thesis/0201294769376.html">人体中可卡因代谢物:尿液中四种未报道过的可卡因代谢物的鉴定</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=孙亚娟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 孙亚娟</a> <span> <a href="/journal-cn-293/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 法医学杂志 </a> </span> <span> . 1991</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-chromatography_thesis/0201246943960.html">超高效液相色谱/飞行时间质谱法分析尿液中的代谢物用于区分人类性别的研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=卢果&option=202" target="_blank" rel="nofollow" class="tuijian_auth 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