Determination of estrone and 17α‐ethinylestradiol in digested sludge by ultrasonic liquid extraction and high‐performance liquid chromatography with fluorescence detection

Louros Vitória L.; Lima Diana L.D.; Leit?o Jorge H.; Esteves Valdemar I.; Nadais Helena G.

摘要

Abstract >Estrone, 17β‐estradiol and 17α‐ethinylestradiol are increasingly recognised as important micropollutants to be monitored in wastewater treatment plants. These estrogens are retained onto sludge due to their high adsorption and since they are largely used in land applications, the monitoring of these chemicals in sludge samples is of great importance. This study describes a method for the determination of estrone and 17α‐ethinylestradiol in fresh sludge samples. After spiking fresh digested sludge with estrone and 17α‐ethinylestradiol and maintaining in contact during 5, 30 and 60?min, the freeze‐dried samples were subjected to ultrasonic liquid extraction, with methanol and acetone, and analysed by high‐performance liquid chromatography with fluorescence detection. The average recoveries obtained for estrone and 17α‐ethinylestradiol using the different contact times were 103?±?3 and 97?±?4%, respectively. Fresh sludge samples from one waste water treatment plant located in Portugal were analysed and estrone was detected in primary fresh sludge, anaerobic digested sludge and dehydrated sludge at a concentration in the range of 1–4.8?μg/g. The method here developed does not require any sample clean‐up, being fast and simple, reliable and inexpensive, making possible its application for monitoring the contamination of sludge with these estrogens. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> > Estrone，17β-雌二醇和17α-乙烯醇雌二醇越来越被认为是要监测的重要微渗透剂污水处理厂。由于其高吸附，这些雌激素保留在污泥上，并且由于它们在很大程度上用于土地应用，因此在污泥样品中监测这些化学品具有重要意义。该研究描述了一种用于测定新鲜污泥样品中的雌激素和17α-乙烯雌二醇的方法。在用雌酮和17α-乙烯烯醇二醇掺入新鲜消化污泥并在5,30和60℃保持接触后，将冷冻干燥的样品进行超声液萃取，用甲醇和丙酮进行超声液萃取，并通过高性能液相色谱法分析荧光检测。使用不同的接触时间为雌激素和17α-乙烯雌二醇获得的平均回收率分别为103Ω·？3和97？±4％。分析了位于葡萄牙的一个废水处理厂的新鲜污泥样品，并在初级新鲜污泥，厌氧消化污泥和脱水污泥中检测到浓度为1-4.8ΩΩ·克/克。这里开发的方法不需要任何样品清理，快速简单，可靠且廉价，使其应用于监测这些雌激素的污泥污染。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2019年第8期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Louros Vitória L.&option=202" target="_blank" rel="nofollow">Louros Vitória L.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lima Diana L.D.&option=202" target="_blank" rel="nofollow">Lima Diana L.D.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Leit?o Jorge H.&option=202" target="_blank" rel="nofollow">Leit?o Jorge H.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Esteves Valdemar I.&option=202" target="_blank" rel="nofollow">Esteves Valdemar I.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nadais Helena G.&option=202" target="_blank" rel="nofollow">Nadais Helena G.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>CESAM &</p> <p>Department of Environment and PlanningUniversity of AveiroAveiro Portugal;</p> <p>CESAM &</p> <p>Department of ChemistryUniversity of AveiroAveiro Portugal;</p> <p>iBB‐Institute for Bioengineering and BiosciencesUniversidade de LisboaLisboa Portugal;</p> <p>CESAM &</p> <p>Department of ChemistryUniversity of AveiroAveiro Portugal;</p> <p>CESAM &</p> <p>Department of Environment and PlanningUniversity of AveiroAveiro Portugal;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=estrogens&option=203" rel="nofollow">estrogens;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=high‐performance liquid chromatography&option=203" rel="nofollow">high‐performance liquid chromatography;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=sludge&option=203" rel="nofollow">sludge;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=sorption&option=203" rel="nofollow">sorption;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ultrasonic liquid extraction&option=203" rel="nofollow">ultrasonic liquid extraction;</a> </p> <div class="translation"> 机译：雌激素;高效液相色谱;污泥;吸附;超声液提取; </div> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div 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