HISTOCHEMICAL DISTRIBUTION OF THREE TYPES OF ENZYMES AND MUCOUS CELLS IN THE GILL, MANTLE AND HEPATOPANCREAS OF THE ARK SHELL SCAPHARCA SUBCRENATA

摘要

The present study was aimed at evaluating the histochemical distribution of alkaline phosphatase (ALP), nonspecific esterase (NSE), peroxidase (POD) and various types of mucous cells in the gill, mantle and hepatopancreas of the ark shell Scapharca subcrenata. The ALP activity was found in the epithelial cells (EP) of the gill filament and the mantle as well as in the basal lamina of the glandular duct in the hepatopancreas. The staining intensity of ALP in the mantle and hepatopancreas was stronger than that in the gill. The NSE activity was detected in the EP in the gill filament, mantle and hepatopancreas, with the hepatopancreas showing a stronger intensity than the gill. The POD activity was located in the EP of the mantle and glandular duct of the hepatopancreas as well as in the connective tissue of the gill. The mantle showed stronger staining intensity of POD than the hepatopancreas. The Alcian blue (pH 2.5) periodic acid Schiff (AB-PAS) histochemical results revealed four types of mucous cells in the gill, mantle and hepatopancreas. Type DI cells (nPAS+AB+) and type IV cells (aPAS+AB-) were observed in the gill, mantle and hepatopancreas. Type I cells (PAS+AB-) were not detected in the hepatopancreas but were distributed ubiquitously throughout the gill filament and mantle. Type II cells (PAS-AB+) were not detected in the gill but were distributed ubiquitously in the digestive ducts of the hepatopancreas.