Analysis of Vitamin B_(12) in Food by Silica Gel 60 TLC and Bioautography with Vitamin B_(12)-Dependent Eschevichia coli 215

摘要

Abstract- To evaluate whether certain foods contain vitamin B_(12) or inactive corrinoids, a simple technique, bioautography with vitamin B_(12)-dependent Escherichia coli mutant after separation of the sample by silica gd 60 thin-layer chromatography, is available. By using the method vita~(TM) B_(12)-compounds found in some edible cyanobacteria are readily identified. This bioautography has great advantages (simplicity, speed, and inexpensiveness) for the analysis of vitamin B_(12)compounds in food.