Determining the Contents of Rupestonic Acid, Vitexicarpin, Apigenin, and Luteolin in Artemisia rupestris L. in Different Growth Stages by Thin-Layer Chromatographic Scanning

摘要

The contents of the four components in different growth stages of Artemisia rupestris L. was determined by thin-layer chromatographic scanning. Chloroform, methanol, formic acid, and water with the proportion of 6.35:0.63:0.17:0.07 were used as the developing solvent. The temperature was 28-32 degrees C. The humidity was less than 30%. The scanning wavelengths were 250 nm and 352 nm. The linearity ranges of rupestonic acid, vitexicarpin, apigenin, and luteolin were 0.128-0.725, 0.0706-0.400, 0.0255-0.145, and 0.0172-0.0976 mu g, respectively. The components had the corresponding correlation coefficients of 0.9909, 0.9908, 0.9971, and 0.9934, which showed a good linear relationship. Precision analyses showed a relative standard deviation of 5.0%. Stability studies showed that rupestonic acid, vitexicarpin, apigenin, and luteolin can be stable for at least 30 min at room temperature. Their average recovery percentages were 99.9%, 103.6%, 98.07%, and 99.9%, respectively. The contents of the four components in the different growth stages of A. rupestris L. were studied. The obvious changes of the four components could be used as the control for the quality of A. rupestris L. and the determination of the time of harvest.