Polyphenolics Free DNA Isolation from Different Types of Tissues of Aconitum heterophyllum Wall-endangered Medicinal Species

摘要

The present study is aimed to isolate DNA from three types of tissues of Aconitum heterophyllum Wall using previously reported methods after modifications. Aconitum heterophyllum Wall is a vital and critically endangered herb, which needs to have itsfingerprinting data for its shrewd use. Use of DNA markers with the techniques like Polymerase Chain Reaction (PCR) and molecular cloning can provide every minute detail about the subject sample. Good quality DNA is always a prerequisite in all molecularbiology experiments especially PCR. As this plant contains high amounts of secondary metabolites that interfere with DNA isolation. The two methodologies described in the present study yielded good-quality, high-molecular weight DNA that is free of contaminants and colored pigments and is suitable for PCR amplification.