Volatile Organic Compounds of Bacillus licheniformis and Ochrobactrum tritici as a Possible Mechanism of Action to Manage Pomegranate Fungal Fruit Rot Pathogen Lasiodiplodia pseudotheo-bromae

摘要

Symptoms of fruit rot were characterized with black necrotic spots of irregular in shape with yellow halo. The spots were surrounded with yellow halo. The lesions were restricted to the fruit skin . Based on the phenotypic and molecular characterization, the pathogen was confired as Lasiodiplodia pseudotheobromae bearing the accession number MG578440. The identified bacterial antagonistic species effective against L. pseudotheobromae were Bacillus licheniformis - strain PB1 (MH470474), Bacillus altitudinus - strain PB2 (MH470475), Bacillus amyloliquefaciens - strain PB3 (MH470473), Ochrobactrum tritici - strain PB8 (MH470478), Bacillus sonarensis - strain PB6 (MH470477) and Bacillus aryabhattai - strain PB7 (MH470480). Among the tested antagonists,the area of mycelial growth of the pathogen L. pseudotheobromae was the minimum with antagonistic bacteria O. tritici followed by B. licheniformis. The antimicrobial compounds associated with B. licheniformis, isolate (PB1) were identified as Acetyl chloride, lH-Pyrrole-2-carboxylic acid, 2-Propenoic acid, 3-phenyl, Phenol, 2,4-bis (1,1-dimethylethyl), Phthalic acid, butyl hept-4-yl ester, 17-Pentatriacontene, Oleic acid, eicosyl ester, Phthalic acid, di(2-propylpentyl) ester. The compounds associated with O. tritici isolate (PB8) were identified as Phosgene, lH-Pyrrole-2-carboxylic acid, Benzenepropanoic acid 1-methylethyl ester, 2-Propenoic acid, 3-phenyl, Phenol, 2,4-bis( 1,1-dimethylethyl), 1,4-Benzenediol, 2,6-bis( 1,1-dimethylethyl), 1-Octade.canol, Phthalic acid, 6-ethyl-3-octyl butyl ester, 12-Oxotetracyclo [5.3.1.1(2,6).0(3,5)]dodec-8-ene, ll-acetoxy-4,4,9-trichloro, 17-Pentatriacontene, Oleic acid, eicosyl ester. Thus these volatile organic compounds might have contributed for the suppression of fruit rot pathogen L. pseudotheobromae.