Medium optimization based on the metabolic-flux spectrum of recombinant Escherichia coli for high expression of human-like collagen II

摘要

Recombinant Escherichia coli BL21 was used to produce human-like collagen II in fed-batch cultivation. By performing MFA (metabolic-flux analysis), the carbonitrogen molar ratios in both the batch and feedingmedia were optimized for high-level production of human-like collagen II. Three carbonitrogen molar ratios in both the batch and feeding media were used in the present study, and the MFA results showed that the optimal carbonitrogen molar ratios for the batch and feeding media were 2.36:1 and 5.12:1 respectively, yielding the highest dry-cell density (67.2 g/l dry cell weight) and human-like collagen production (10.8 g/l).