Conversion of Linoleic Acid into 10-Hydroxy-12(Z)-octadecenoic Acid by Whole Cells of Stenotrophomonas nitritireducens

摘要

The conversion of linoleic acid into 10-hydroxy-12(Z)-octadecenoic acid by whole cells of Stenotrophomonas nitritireducens as an isolated bacterium was optimized,and the optimal temperature,pH,and cell and substrate concentrations were 30 °C,7.5,and 20 and 20 g/L,respectively.Under these conditions,whole cells in a bioreactor produced 15 g/L 10-hydroxy-12(Z)-octadecenoic acid in 2 h of reaction time without detectable byproducts.Using 2 g/L linoleic acid,the cells produced 1.92 g/L 10-hydroxy-12(Z)-octadecenoic acid.These are the highest concentration and yield of 10-hydroxy-12(Z)-octadecenoic acid ever reported.