...
首页> 外文期刊>Journal of Functional Foods >Toward an accessible and robust in vitro approach to evaluate bacterial viability in the upper gastro-intestinal tract: A Gastro-Intestinal Digestive Simulator (GIDS) combined with alternative methods to plating
【24h】

Toward an accessible and robust in vitro approach to evaluate bacterial viability in the upper gastro-intestinal tract: A Gastro-Intestinal Digestive Simulator (GIDS) combined with alternative methods to plating

机译:朝着可进入和稳健的体外方法来评估上胃肠道中的细菌活力:胃肠消化模拟器(GID)与替代方法相结合电镀

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       

摘要

Since the viability throughout the human gastro-intestinal (GI) tract is a key metric to select new probiotics, we aimed to develop an evolutive and robust semi-dynamic model, named Gastro-Intestinal Digestive Simulator (GIDS), mimicking pH variations of the upper GI tract and based on the latest Infogest in vitro digestion protocol recommendations. We first tested the GIDS and its reproducibility by measuring, during in vitro digestion, the cultivability of bacterial strain contained in two marketed fermented milk products. Then, we used this model to assess inter- and infra-species variability among 16 bacterial strains from Bifidobacterium animalis, Bifidobacterium breve, and Lactobacillus paracasei species. Finally, we further explored the applicability of propidium monoazide-quantitative PCR and flow cytometry to GIDS samples. To summarize, our study showed that i) the GIDS model is reproducible and ii) the complementary methods to plating tested here are compatible with GI environments; providing insights that will guide future developments.
机译:由于整个人胃肠(GI)道的可行性是选择新益生菌的关键指标,我们旨在开发一种演变和鲁棒的半动态模型,名为胃肠消化模拟器(GID),模仿pH变化上GI道,并基于最新的含有体外消化议定书协议的建议。我们首先通过在体外消化期间测量了GID和其再现性,在体外消化中,两种销售发酵乳产品中包含的细菌菌株的可培养性。然后,我们利用该模型评估来自动物,Bifidobacterium Breve和乳杆菌乳菌物种的16种细菌菌株之间的16种细菌菌株之间的互变性。最后,我们进一步探索了单氮杂化定量PCR和流式细胞术中的适用性与GIDS样品。总而言之,我们的研究表明,I)GIDS模型可重现,II)此处测试的互补方法与GI环境兼容;提供将引导未来发展的见解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号