Bovine alpha-lactalbumin hydrolysates (alpha-LAH) attenuate high-fat diet induced nonalcoholic fatty liver disease by modulating hepatic lipid metabolism in C57BL/6J mice

摘要

The effect of bovine alpha-lactalbumin hydrolysates (alpha-LAH) on attenuating high-fat diet (HFD) induced nonalcoholic fatty liver disease (NAFLD) was investigated. C57BL/6J mice were fed with HFD for 8 weeks to mimic the clinical features of NAFLD, and then were fed with HFD and bovine alpha-LAH at different doses (100, 200 and 400 mg/kg b.w.) for another 12 weeks to investigate the beneficial effects of bovine alpha-LAH on NAFLD. Our data showed that bovine alpha-LAH markedly reversed the increase of body weight gain, tissue weight, serum lipids, liver injury markers, and serum pro-inflammatory cytokines induced by HFD in C57BL/6J mice. Bovine alpha-LAH supplementation also ameliorated fat accumulation and oxidative stress, down-regulated PPAR gamma associated gene expression, and up-regulated PPAR alpha associated gene expression in the liver of mice fed HFD. These findings suggested that bovine alpha-LAH is effective in attenuating HFD induced NAFLD in C57BL/6J mice.