THE INFLUENCE OF ELEVATED OZONE CONCENTRATION IN AIR ON SELECTED BIOCHEMICAL PARAMETERS, HEMATOLOGICAL INDICES AND IMMUNOLOGICAL PARAMETERS IN RATS FED WITH VARIOUS VITAMIN E AND C CONCENTRATIONS: II. The influence of the elevated ozone concentration

摘要

Three separate experiments (E, E+C and C) were carried out on 180 Wistar/Hannower rats. The rats were divided into six groups in each experiment depending on their exposure and the vitamin E and C supplement in the standard feed. The control animalsand the rats from group II were fed the standard NMurigranO feed for rodents but animals in groups III--VI were fed feed enriched with vitamin E, E+C or C. The experimental animals from groups II-VI were exposed to l.0ń0.4 mg O_3/m~3 air for 8 hours a day over 5 weeks. After the experiment, the rat lungs were separated, lung tissue was homogenised and supernatant was obtained. The gas chromatography technique was used to determine the share of mono- and polyunsaturated fatty acids in the lung tissue. The lipid and water percentage in the lipid peroxidation product fluorescence level, and the concentrations of malonyldialdehyde and vitamin C were determined with the use of the spectrophotometric technique. However, the total protein was determined in the homogenised lung tissue supernatant. The results were analysed statistically. A negative ozone influence on the rat lungs was observed resulting in the intensification of lipid peroxidation processes. Based on the experiments, it may be concluded thatfeeding animals with the feed supplemented with vitamins E and C is beneficial for rats when exposed to air with elevated ozone concentrations.