Coreopsis tinctoria flowers extract ameliorates D-galactose induced aging in mice via regulation of Sirtl-Nrf2 signaling pathway

摘要

The aim of this study was to investigate the protective effects and its underlying signaling pathways of Coreopsis tinctoria flowers extract (CTE) on D-galactose induced aging in mice. UPLC/Q-TOF-MS analysis of CTE identified 12 active compounds, including three phenolic acids and nine flavonoids. The extract showed superior antioxidant activities in vitro using DPPH, FRAP, and ORAC assay. Administration with CTE remarkably improved body weight, organ indexes of aging mice, and liver histological injury as well as decreased oxidative stress markers levels of aged mice. Further studies showed CTE treatment significantly enhanced Sirtl expression and activated the Nrf2 signaling pathway by the increase of Nrf2 nuclear translocation, NQO-1 and HO-1 expression. Furthermore, CTE treatment resulted in the increase of Sirt3 and the suppression of p53. These results demonstrated that the promising anti-aging effects of Coreopsis tinctoria flowers extract might be related to up regulation of Sirtl-Nrf2 signaling pathway.