Uncovering matrix effects on lipid analyses in MALDI imaging mass spectrometry experiments

Perry William J.; Patterson Nathan Heath; Prentice Boone M.; Neumann Elizabeth K.; Caprioli Richard M.; Spraggins Jeffrey M.

摘要

Abstract >The specific matrix used in matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) can have an effect on the molecules ionized from a tissue sample. The sensitivity for distinct classes of biomolecules can vary when employing different MALDI matrices. Here, we compare the intensities of various lipid subclasses measured by Fourier transform ion cyclotron resonance (FT‐ICR) IMS of murine liver tissue when using 9‐aminoacridine (9AA), 5‐chloro‐2‐mercaptobenzothiazole (CMBT), 1,5‐diaminonaphthalene (DAN), 2,5‐Dihydroxyacetophenone (DHA), and 2,5‐dihydroxybenzoic acid (DHB). Principal component analysis and receiver operating characteristic curve analysis revealed significant matrix effects on the relative signal intensities observed for different lipid subclasses and adducts. Comparison of spectral profiles and quantitative assessment of the number and intensity of species from each lipid subclass showed that each matrix produces unique lipid signals. In positive ion mode, matrix application methods played a role in the MALDI analysis for different cationic species. Comparisons of different methods for the application of DHA showed a significant increase in the intensity of sodiated and potassiated analytes when using an aerosol sprayer. In negative ion mode, lipid profiles generated using DAN were significantly different than all other matrices tested. This difference was found to be driven by modification of phosphatidylcholines during ionization that enables them to be detected in negative ion mode. These modified phosphatidylcholines are isomeric with common phosphatidylethanolamines confounding MALDI IMS analysis when using DAN. These results show an experimental basis of MALDI analyses when analyzing lipids from tissue and allow for more informed selection of MALDI matrices when performing lipid IMS experiments. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >在基质辅助激光解吸/电离成像质谱（MALDI IMS）中使用的特定基质可以对从组织样品电离的分子产生效果。当采用不同的MALDI矩阵时，不同类别的生物分子的敏感性可以变化。在这里，我们在使用9-氨基吖啶（9AA），5-氯-2-巯基苯噻唑（CMBT），1,5时，将傅里叶变换离子回旋谐振（FT-ICR）IMS测量的各种脂质亚类的强度进行比较。 ---亚胺萘（DAN），2,5-二羟基乙酮（DHA）和2,5-二羟基苯甲酸（DHB）。主要成分分析和接收器操作特征曲线分析显示对不同脂质亚类和加合物观察到的相对信号强度的显着基质效应。从每种脂质亚类的谱分布和测量物种数量和定量评估的比较显示，每个基质产生独特的脂质信号。在阳性离子模式中，基质施用方法在不同阳离子种类的MALDI分析中发挥了作用。 DHA应用的不同方法的比较显示使用气溶胶喷雾器时的链接和钾分析物强度的显着增加。在负离离子模式中，使用丹产生的脂质型材显着不同于所测试的所有其他基质。发现这种差异是通过电离期间的磷脂酰胆碱的改变而驱动，使得它们能够以负离子模式检测。这些改性的磷脂酰胆碱是异构的，常用磷脂酰乙醇胺在使用丹时混淆MALDI IMS分析。这些结果表明，当分析来自组织的脂质时，MALDI分析的实验基础，并在进行脂质IMS实验时允许更明智地选择MALDI矩阵。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS 》</a> <b style="margin: 0 2px;">|</b><span>2020年第4期</span><b style="margin: 0 2px;">|</b> <span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Perry William J.&option=202" target="_blank" rel="nofollow">Perry William J.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Patterson Nathan Heath&option=202" target="_blank" rel="nofollow">Patterson Nathan Heath;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Prentice Boone M.&option=202" target="_blank" rel="nofollow">Prentice Boone M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Neumann Elizabeth K.&option=202" target="_blank" rel="nofollow">Neumann Elizabeth K.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Caprioli Richard M.&option=202" target="_blank" rel="nofollow">Caprioli Richard M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Spraggins Jeffrey M.&option=202" target="_blank" rel="nofollow">Spraggins Jeffrey M.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Mass Spectrometry Research CenterVanderbilt UniversityNashville Tennessee;</p> <p>Mass Spectrometry Research CenterVanderbilt UniversityNashville Tennessee;</p> <p>Mass Spectrometry Research CenterVanderbilt UniversityNashville Tennessee;</p> <p>Mass Spectrometry Research CenterVanderbilt UniversityNashville Tennessee;</p> <p>Mass Spectrometry Research CenterVanderbilt UniversityNashville Tennessee;</p> <p>Mass Spectrometry Research CenterVanderbilt UniversityNashville Tennessee;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=imaging mass spectrometry&option=203" rel="nofollow">imaging mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lipid imaging&option=203" rel="nofollow">lipid imaging;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=MALDI IMS&option=203" rel="nofollow">MALDI IMS;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=matrix‐assisted laser desorption/ionization mass spectrometry&option=203" rel="nofollow">matrix‐assisted laser desorption/ionization mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=MALDI matrix&option=203" rel="nofollow">MALDI matrix;</a> </p> <div class="translation"> 机译：成像质谱;脂质成像;MALDI IMS;矩阵辅助激光解吸/电离质谱;MALDI矩阵; 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href="/academic-journal-cn_chinese-journal-analytical-chemistry_thesis/0201230466410.html">金属/基质增强飞行时间-二次离子质谱用于单细胞脂质分析</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李好问&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李好问</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=华鑫&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,华鑫</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=龙亿涛&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,龙亿涛</a> <span> <a href="/journal-cn-10804/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析化学 </a> </span> <span> . 2018</span><span> ,第001期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-analytical-chemistry_thesis/0201230482248.html">基于离子液体基质的大豆中寡糖成分基质辅助激光解吸电离-质谱成像分析</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=裴兴丽&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 裴兴丽</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄煜宇&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,黄煜宇</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=龚灿&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,龚灿</a> <span> <a href="/journal-cn-10804/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析化学 </a> </span> <span> . 2017</span><span> ,第008期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-clinical-hepatology_thesis/0201279860513.html">靶向脂质组学揭示急性失代偿期肝硬化患者循环脂质介体的广泛变化</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=马仁舒&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 马仁舒</a> <a 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<span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=汪曼&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 汪曼</a> <span> . 2019</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120113143105.html">复杂基质中分析物的定量质谱分析中基质效应校正的方法</a> <b>[P]</b> . <span> 中国专利： CN113196052A </span> <span> . 2021-07-30</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061204279546.html">使用了质谱分析的脂质解析方法和质谱分析装置</a> <b>[P]</b> . <span> 中国专利： CN109148257B </span> <span> . 2021.03.09</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130412488232.html">Matrix assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI)</a> <b>[P]</b> . <span> 外国专利：  US9261438B2 </span> <span> . 2016-02-16</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基质辅助激光解吸电离质谱成像（MALDI-MSI） </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130418254467.html">Matrix Assisted Laser Desorption Ionisation Mass Spectrometry Imaging (MALDI-MSI)</a> <b>[P]</b> . <span> 外国专利：  US2014084151A1 </span> <span> . 2014-03-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基质辅助激光解吸电离质谱成像（MALDI-MSI） </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130417075670.html">IMPROVEMENTS TO MATRIX ASSISTED LASER DESORPTION IONISATION MASS SPECTROMETRY IMAGING (MALDI-MSI)</a> <b>[P]</b> . <span> 外国专利：  EP2684021A1 </span> <span> . 2014-01-15</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: 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