Quaternary ammonium isobaric tag for a relative and absolute quantification of peptides

Setner Bartosz; Stefanowicz Piotr; Szewczuk Zbigniew

摘要

Abstract >Isobaric labeling quantification of peptides has become a method of choice for mass spectrometry‐based proteomics studies. However, despite of wide variety of commercially available isobaric tags, none of the currently available methods offers significant improvement of sensitivity of detection during MS experiment. Recently, many strategies were applied to increase the ionization efficiency of peptides involving chemical modifications introducing quaternary ammonium fixed charge. Here, we present a novel quaternary ammonium–based isobaric tag for relative and absolute quantification of peptides (QAS‐iTRAQ 2‐plex). Upon collisional activation, the new stable benzylic‐type cationic reporter ion is liberated from the tag. Deuterium atoms were used to offset the differential masses of a reporter group. We tested the applicability of QAS‐iTRAQ 2‐plex reagent on a series of model peptides as well as bovine serum albumin tryptic digest. Obtained results suggest usefulness of this isobaric ionization tag for relative and absolute quantification of peptides. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >等离标签肽的定量已成为质谱基型蛋白质组学研究的选择方法。然而，尽管有各种各样的商用等离性标签，但目前可用的方法都不是在MS实验期间显着提高检测灵敏度。最近，应用了许多策略来提高涉及季铵固定电荷的化学修饰的肽的电离效率。在这里，我们提出了一种用于肽的相对和绝对定量的新型季铵盐的等离性标签（Qas-Itraq 2-Plex）。在碰撞激活时，从标签中释放出新的稳定苄基型阳离子报告离子。氘原子用于抵消报告组的差分肿块。我们在一系列模型肽和牛血清白蛋白胰蛋白酶消化中测试了Qas-Itraq 2-Plex试剂的适用性。获得的结果表明，这种同学电离标签的有用性，用于肽的相对和绝对定量。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS》</a> <b style="margin: 0 2px;">|</b><span>2018年第2期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Setner Bartosz&option=202" target="_blank" rel="nofollow">Setner Bartosz;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Stefanowicz Piotr&option=202" target="_blank" rel="nofollow">Stefanowicz Piotr;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Szewczuk Zbigniew&option=202" target="_blank" rel="nofollow">Szewczuk Zbigniew;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Faculty of ChemistryUniversity of Wroc?awF. Joliot‐Curie 14 Wroc?aw 50‐383 Poland;</p> <p>Faculty of ChemistryUniversity of Wroc?awF. Joliot‐Curie 14 Wroc?aw 50‐383 Poland;</p> <p>Faculty of ChemistryUniversity of Wroc?awF. Joliot‐Curie 14 Wroc?aw 50‐383 Poland;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=derivatization of peptides&option=203" rel="nofollow">derivatization of peptides;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=isobaric tags&option=203" rel="nofollow">isobaric tags;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=peptide sequencing&option=203" rel="nofollow">peptide sequencing;</a> </p> <div class="translation"> 机译：肽的衍生化;表征标签;肽测序; 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