Phytoestrogens in soy‐based meat substitutes: Comparison of different extraction methods for the subsequent analysis by liquid chromatography‐tandem mass spectrometry

Benedetti Barbara; Di Carro Marina; Magi Emanuele

摘要

Abstract >This paper presents the development of an efficient extraction procedure followed by a high performance liquid chromatography‐tandem mass spectrometry (HPLC‐MS/MS) method for the determination of five phytoestrogens (genistein, daidzein, formononetin, biochanin A, and coumestrol) in soy‐based meat substitutes. Phytoestrogens are considered endocrine disrupting compounds, and their quantification is important in soy‐based products, whose diffusion is increasing nowadays. >The HPLC‐MS/MS method, with electrospray ionization (ESI) source, was optimized to obtain high specificity and sensitivity, as well as rapidity of the analysis. Three extraction techniques were applied to soy burgers and compared: ultrasound assisted extraction, ultrasound assisted extraction followed by solid phase extraction and the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) methodology. Both ultrasound assisted extraction and QuEChERS proved to be suitable for the determination of phytoestrogens, showing high recoveries, in the range of 86% to 99% and 75% to 105%, respectively. Matrix effect was evaluated, and ion suppression was observed for coumestrol and formononetin, demonstrating the importance of matrix effect assessment when complex samples are analyzed by HPLC‐ESI‐MS. > The complete analytical protocols provided limits of detection and quantitation in soy‐burgers at the ng g ?1 level for all the considered phytoestrogens. Some soy burger samples were analyzed by both ultrasound assisted extraction and QuEChERS followed by HPLC‐MS/MS. High concentration levels of daidzein and genistein (2‐59?μg?g ?1 and 2‐72?μg?g ?1 , respectively) were found; formononetin was in the range of 5 to 26?ng?g ?1 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >本文呈现高效液相色谱 - 串联质谱（HPLC-MS / MS）测定大豆基于大豆的五种植物雌激素（HPLC-MS / MS）方法的高效液相色谱 - 串联质谱（HPLC-MS / MS）方法。肉类替代品。植物雌激素被认为是内分泌破坏化合物，它们的定量在基于大豆的产品中是重要的，其扩散现在增加。HPLC-MS / MS方法，用电喷雾电离（ESI）源进行了优化获得高特异性和敏感性，以及分析的速度。将三种提取技术应用于大豆汉堡，并比较：超声辅助提取，超声辅助提取，然后是固相萃取和快速容易廉价的有效崎岖和安全（Quechers）方法。超声辅助提取和QueChers被证明是适用于植物雌激素的测定，显示高回收率，分别为86％至99％和75％至105％。评价基质效应，对COUMESTROL和Formononetin观察到离子抑制，当通过HPLC-ESI-MS分析复杂样品时，表明基质效应评估的重要性。</ p> >完整的分析协议提供了检测限制所有被认为的植物雌激素的NG G <SUP>β1</ sup>水平的大豆汉堡中的定量。通过超声辅助提取和QueChers分析一些大豆汉堡样品，然后通过HPLC-MS / MS进行分析。发现高浓度水平的DaIdezein和Genistein（2-59〜μg≤1μg> 1 / sop>和2-72×1/72Ω，分别）; Formononetin的范围为5至26ΩNG≤G？1 </ sup> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS 》</a> <b style="margin: 0 2px;">|</b><span>2018年第9期</span><b style="margin: 0 2px;">|</b> <span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Benedetti Barbara&option=202" target="_blank" rel="nofollow">Benedetti Barbara;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Di Carro Marina&option=202" target="_blank" rel="nofollow">Di Carro Marina;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Magi Emanuele&option=202" target="_blank" rel="nofollow">Magi Emanuele;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemistry and Industrial ChemistryUniversity of GenoaVia Dodecaneso 31 16146 Genoa Italy;</p> <p>Department of Chemistry and Industrial ChemistryUniversity of GenoaVia Dodecaneso 31 16146 Genoa Italy;</p> <p>Department of Chemistry and Industrial ChemistryUniversity of GenoaVia Dodecaneso 31 16146 Genoa Italy;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=extraction techniques&option=203" rel="nofollow">extraction techniques;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=liquid chromatography&option=203" rel="nofollow">liquid chromatography;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=phytoestrogens&option=203" rel="nofollow">phytoestrogens;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=QuEChERS&option=203" rel="nofollow">QuEChERS;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=soy‐based food&option=203" rel="nofollow">soy‐based food;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=tandem mass spectrometry&option=203" rel="nofollow">tandem mass spectrometry;</a> </p> <div class="translation"> 机译：提取技术;液相色谱;植物雌激素;Quechers;基于大豆的食物;串联质谱; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025633044.html">Phytoestrogens in soy‐based meat substitutes: Comparison of different extraction methods for the subsequent analysis by liquid chromatography‐tandem mass spectrometry</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Benedetti Barbara&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Benedetti Barbara,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Di Carro Marina&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Di Carro Marina,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Magi Emanuele&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Magi Emanuele </a> <a href="/journal-foreign-34906/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of mass spectrometry: JMS .</a> <span>2018</span> <span>,第9期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：大豆肉类替代品中的植物雌激素：液相色谱 - 串联质谱法随后分析的不同提取方法的比较</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025562560.html">Multiresidue analysis of tetracycline and beta-receptor agonists in chicken by pressurized liquid extraction and liquid chromatography-tandem mass spectrometry: Comparison with QuEChERS extraction method and ultrasound assisted extraction</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Mengting&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wang Mengting,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Peng Bin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Peng Bin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhao Na&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhao Na,</a> <a href="/journal-foreign-34545/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Food Composition and Analysis .</a> <span>2020</span> <span>,第期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过加压液萃取和液相色谱 - 串联质谱法的四环素和β-受体激动剂的多义分析：与QueCher萃取方法的比较和超声辅助提取</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704028337044.html">High-Throughput Mega-Method for the Analysis of Pesticides, Veterinary Drugs, and Environmental Contaminants by Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry and Robotic Mini-Solid-Phase Extraction Cleanup + Low-Pressure Gas Chromatography–Tandem Mass Spectrometry, Part 2: Catfish</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ederina Ninga&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ederina Ninga,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yelena Sapozhnikova&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yelena Sapozhnikova,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Steven J. Lehotay&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Steven J. Lehotay,</a> <a href="/journal-foreign-33544/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Agricultural and Food Chemistry .</a> <span>2021</span> <span>,第4期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：超高效液相色谱 - 串联质谱和机器人迷你固相提取清除+低压气相色谱 - 串联质谱法分析农药，兽医药物和环境污染物的分析。第2部分：鲶鱼</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-257539_thesis/0705010487089.html">High throughput liquid chromatography/tandem mass spectrometry method for quantification of isoflavones, their metabolites and other phytoestrogens</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jeevan K. Prasain&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jeevan K. Prasain,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Alireza Arabshahi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Alireza Arabshahi,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ray Moore&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ray Moore,</a> <a href="/conference-foreign-257539/" target="_blank" rel="nofollow" class="tuijian_authcolor">American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics .</a> <span>2009</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：高通量液相色谱/串联质谱法，用于量化异黄酮，代谢物和其他植物雌激素</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061793123.html">Examination of Concentrations of THC and Its Major Metabolites in Postmortem Blood Using Solid-Phase Extraction Preparation Methods and Analysis by Liquid Chromatography-Tandem Mass Spectrometry</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tully, Andrea Kristin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Tully, Andrea Kristin </a> <span>2018</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：固相萃取制备方法检测死后血液中四氢大麻酚及其主要代谢物的浓度，并通过液相色谱-串联质谱法进行分析</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040005944416.html">Simultaneous determination of eleven phytoestrogens in human serum using a two minute liquid chromatography/tandem mass spectrometry method</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jeevan K. Prasain&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jeevan K. Prasain,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Alireza Arabshahi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Alireza Arabshahi,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=D. Ray Moore II&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">D. Ray Moore II,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：一种使用两分钟液相色谱/串联质谱法同时测定人血清中11植物雌激素</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100016663720.html ">Optimization of clean extraction methods to isolate carotenoids from the microalga Neochloris oleoabundans and subsequent chemical characterization using liquid chromatography tandem mass spectrometry</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Castro-Puyana, M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Castro-Puyana, M.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Herrero, Miguel&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Herrero, Miguel,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Urreta, Iratxe&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Urreta, Iratxe,</a> <span>2014</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：优化清洁提取方法以从微藻新油菜中分离类胡萝卜素并随后使用液相色谱串联质谱法进行化学表征</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/ntis-science-report_pb_thesis/02071380011.html">Methods of Analysis by the U.S. Geological Survey Organic Geochemistry Research Group: Determination of Dissolved Isoxaflutole and Its Sequential Degradation Products, Diketonitrile and Benzoic Acid, in Water Using Solid-Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Meyer, M. T.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Meyer, M. T.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lee, E. A.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lee, E. A.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Scribner, E. A.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Scribner, E. A. </a> <span>2007</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：美国地质调查局有机地球化学研究组分析方法：采用固相萃取和液相色谱/串联质谱法测定水中溶解的异恶唑草酮及其顺序降解产物，二酮腈和苯甲酸</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-analytical-chemistry_thesis/0201230572257.html">高效液相色谱-串联质谱法分析大肠杆菌代谢组中样品提取方法的比较</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=梅辉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 梅辉</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=戴军&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,戴军</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘文卫&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘文卫</a> <span> <a href="/journal-cn-10804/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析化学 </a> </span> <span> . 2011</span><span> ,第004期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-analytical-chemistry_thesis/0201230539586.html">在线固相萃取-高效液相色谱-串联质谱法同时检测大豆不同部位的4种植物激素</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=贾鹏禹&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 贾鹏禹</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曾明飞&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,曾明飞</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=冯乃杰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,冯乃杰</a> <span> <a href="/journal-cn-10804/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析化学 </a> </span> <span> . 2014</span><span> ,第012期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-analytical-chemistry_thesis/0201230596198.html">快速高分离液相色谱-串联质谱法测定植物组织中雌激素玉米赤霉醇类化合物</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张岩&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 张岩</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李挥&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李挥</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张敬轩&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张敬轩</a> <span> <a href="/journal-cn-10804/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析化学 </a> </span> <span> . 2009</span><span> ,第011期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-analytical-chemistry_thesis/0201230574657.html">超高效液相色谱-四极杆飞行时间串联质谱法同时分析奶粉中9种雌激素</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王和兴&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王和兴</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=周颖&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,周颖</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=姜庆五&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,姜庆五</a> <span> <a href="/journal-cn-10804/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析化学 </a> </span> <span> . 2011</span><span> ,第009期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-oil-crop-sciences_thesis/0201281344512.html">基于液相色谱-串联质谱法分析不同植物油经加热处理前后氧化脂肪酸的变化</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=覃佐剑&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 覃佐剑</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=吴宗远&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,吴宗远</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=涂行浩&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,涂行浩</a> <span> <a href="/journal-cn-6915/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国油料作物学报 </a> </span> <span> . 2020</span><span> ,第003期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-4679_thesis/020221136388.html">固相萃取——高效液相色谱串联质谱法测定原料奶中5种环境雌激素残留量的研究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李雪&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李雪</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=牟光庆&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,牟光庆</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈历俊&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈历俊</a> <span> <a href="/conference-cn-4679/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 首届全国奶牛精细化管理高峰论坛暨奶牛精细化饲养关键技术与设施设备研讨会 </a> <span> <span> . 2013</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020314884594.html">固相萃取-超高效液相色谱串联质谱法对杭州市不同水环境中13种痕量药物残留状况的检测及分析</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李晓娟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李晓娟</a> <span> . 2011</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120112569478.html">同时检测血清中3种雌激素的化学衍生-超高效液相色谱-串联质谱法</a> <b>[P]</b> . <span> 中国专利： CN112611827A </span> <span> . 2021-04-06</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120110054163.html">一种固相萃取‑液相色谱串联质谱法同时检测水体中9种雌激素类物质的方法</a> <b>[P]</b> . <span> 中国专利： CN107543876A </span> <span> . 2018-01-05</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130422148767.html">METHOD AND AN APPARATUS FOR CLASSIFYING ARTEMISIA SPECIES AND THE DRIED LEAF OF THE ARTEMISIA ASIATICA CAPABLE OF ACCURATELY ANALYZING THE METABOLOME OF ARTEMISIA BASED ON A GAS CHROMATOGRAPHY/MASS SPECTROMETRY OR LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY METABOLOMICS ANALYSIS</a> <b>[P]</b> . <span> 外国专利：  KR20110125373A </span> <span> . 2011-11-21</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于气相色谱/质谱或液相色谱/质谱分析法对蒿属菌种和干蒿进行分类的方法和装置，能够准确地分析蒿属的代谢 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130405948035.html">LC-MS/MS Discrimination Method of Geographical Origin for Commercial Red Ginseng concentrate Using combined Liquid Chromatography-tandem mass spectrometer/MS and Electronic Nose based on mass spectrometry</a> <b>[P]</b> . <span> 外国专利：  KR101817824B1 </span> <span> . 2018-01-12</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：液相色谱-串联质谱/ MS和基于质谱的电子鼻相结合的商品红参浓缩液的LC-MS / MS地理来源鉴别方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130411503880.html">DISCRIMINATION METHOD OF GEOGRAPHICAL ORIGIN FOR COMMERCIAL RED GINSENG CONCENTRATE USING COMBINED LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETER/MS AND ELECTRONIC NOSE BASED ON MASS SPECTROMETRY</a> <b>[P]</b> . <span> 外国专利：  KR20160053101A </span> <span> . 2016-05-13</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：液相色谱-串联质谱/质谱结合质谱法鉴别红参精矿的地理来源 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025633044','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704025633044down" data-source="7," data-out-id="b4B9yYblxPOS7Im37b2R4hwaISxWshhk96iZEVqYupc8Mxit7kqWOzgZms/zkjkP," data-f-source-id="7" data-title="Phytoestrogens in soy‐based meat substitutes: Comparison of different extraction methods for the subsequent analysis by liquid chromatography‐tandem mass spectrometry" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="34906" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Phytoestrogens in soy‐based meat substitutes: Comparison of different extraction methods for the subsequent analysis by liquid chromatography‐tandem mass spectrometry"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.9"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.9"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.9"></script> </body> <script> function kefu(customization) { var from = GetQueryString("from"); if (from == null || from == "") { from = "other"; } var url = getWxWorkQrCode(55, from, customization); if (url == "" || typeof url == 'undefined') { url = "https://wework.qpic.cn/wwpic/994838_0LUTS-VqR0quT1N_1675665967/0"; } tipDocGuidance(url, '人工辅助获取外文期刊材料'); } $(function () { var weiPuStatus = getCookie('WeiPuStatus'); if (weiPuStatus) { tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if ((sourcetype == 1 || sourcetype == 4) && inyn_provide_service_level != 4) { getJournal(); } }) </script> </html>