Receptor targeted lymphoscintigraphy using dextran derivatives labelled with~(99m)TC: In vivo preliminary biological evaluation

摘要

The radiolabelling of mannosyl-dextran derivatives with ~(99m)Tc resulting in a high purity and stability radiolabelled conjugate, suitable for sentinel node detection with low distal lymph accumulation, as well as their in vivo biological evaluation are the proposed aims of this study. Different radiolabelling strategies, including novel ~(99m)Tc cores, were evaluated in order to select and optimize the most efficient and specific one. The analysis of radiolabeled conjugates using TLC and HPLC was performed; the RCPs of the probes were ranged between 93-99%. The biological evaluation (ex-vivo biodistribution and specific uptake) was performed in Wistar rats at 15, 30 and 60 min post injection. The biological data shows a rapid and highly specific sentinel node accumulation, up to 7.5% ID, and a very good sentinel node extraction in respect with the second node in the chain up to 83% at 1h p.i.