The chemistry and engineering of mussel-inspired glue matrix for tissue adhesive and hemostatic

摘要

The present study aimed to develop a bio-adhesive material with excellent hemostatic and rapid wound healing properties using a catechol moiety (2chloro-3',4'-dihydroxyacetophenone) conjugated to poly (dimethyl amino ethyl methacrylate-co-t-butyl methacrylate) poly(DMA-co-t-BMA)-2-chloro-3',4'dihydroxyacetophenone (CA), C-PDB]. The desired glue matrix was formed by crosslinking between CPDB and chitosan via the Michael addition reaction. During glue matrix formation, chitosan plays a vital role as a cross-linker because the amine group of chitosan can easily react with the quinone from CA, based on catechol chemistry under mild alkaline conditions. Lap shear strength tests showed that the formulated glue matrix had a significant adhesive force at around 1.288 MPa and it formed quickly in vitro. In addition, when applied on a bleeding site on a rat aorta in vivo, the prepared glue matrix displayed strong hemostatic behavior. Hematoxylin and eosin (H&E), Masson's trichrome, and reticulin staining of wounds (hepatic tissue) treated with the prepared glue matrix displayed rapid wound healing. Therefore, the prepared glue matrix is a potential candidate as a hemostatic and wound-healing agent for clinical applications. (C) 2019 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.