Evaluation of Anyplex (TM) II RV16 and RB5 real-time RT-PCR compared to Seeplex (R) RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens

摘要

This prospective study was performed to evaluate and compare the performance of the multiplex PCR Seeplex (R) assays and Anyplex (TM) II assays. From May 2014 until April 2016, a total of 247 respiratory samples were collected in Okinawa, Japan. Multiple respiratory pathogens were detected in 37% of patients with positive results. The most prevalent pathogens were influenza A virus and respiratory syncytial virus B. Despite minor differences in capabilities, both the Seeplex (R) assays and Anyplex (TM) II assays can be easily implemented in diagnostic or research laboratories to optimize the detection and management of respiratory pathogen induced diseases. (C) 2017 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.