Nephroprotective effect of Paeonia emodi via inhibition of advanced glycation end products and oxidative stress in streptozotocin–nicotinamide induced diabetic nephropathy

摘要

Abstract The present study aimed to evaluate the effect of alcohol (PA) and hydroalcohol (PHA) extract of Paeonia emodi Royale roots in treatment of streptozotocin–nicotinamide induced diabetic nephropathy. Diabetes mellitus was induced in male Wistar rats by streptozotocin (65?mg/kg intraperitoneally) 15?minutes after nicotinamide (230?mg/kg, intraperitoneally) administration and diabetic nephropathy was assessed by measuring serum glucose, renal parameters (urea, uric acid, creatinine, and blood urea nitrogen level) and lipid profile. The rats were treated with different doses of extracts (100?mg/kg, 200?mg/kg, and 400?mg/kg) for 45?days. Oxidative stress was assessed by measuring tissue antioxidant enzymes level along with the formation of advanced glycation end-products (AGEs) in kidney. PA and PHA (400?mg/kg) produced significant attenuation in the serum glucose level (165.08?±?3.353?mg/dL and 154.27?±?2.209?mg/dL, respectively) as compared to control. Elevated renal parameters, lipid levels, tissue antioxidant enzymes and AGE formation were also restored in a dose-dependent manner. These findings suggest that by amelioration of oxidative stress and formation of AGEs, PA and PHA significantly inhibited the progression diabetic nephropathy in rats. Graphical abstract Display Omitted Highlights ? Paeonia emodi is an erect glabrous, leafy perennial herb indigenous to north-west Himalayas commonly known as Ood-saleeb. ? It can be concluded that P. emodi showed protective effect against diabetic nephropathy. ? P. emodi extracts exhibited beneficial effects on the blood glucose level and associated biomarkers of diabetic nephropathy.