Dansyl Based 'Turn-On' Fluorescent Sensor for Cu2+ Ion Detection and the Application to Living Cell Imaging

摘要

A new "turn-on" fluorescent chemosensor based on dansyl derivative was prepared for Cu2+ ion sensing. Hydroxyl, imine and azomethine groups in Schiff base derived compound 1 were deliberately introduced for facilitating the binding of Cu2+ ion. Of screen metal ions, compound 1 showed a high degree of selectivity toward Cu2+ ion. Other interfering metal ions did not affect the fluorescence intensity of compound 1, except Hg2+ and Fe3+ ions exhibited a significant degree of fluorescence quenching. Upon binding of Cu2+ ion, compound 1 displayed a chelation enhanced fluorescence (CHEF) resulting in increasing of the fluorescence intensity. The molecular optimized geometry indicated the binding ratio between compound 1 and Cu2+ ion at 1:1 with the binding constant of 1.68 x 10(- 7) M- 1. The optimized condition for sensing ability of compound 1 with a detection limit of 5 x 10(- 7) M was found at the physiological pH 7.2 with the excitation wavelength of 366 nm. Due to no cytotoxicity and good photophysical properties, compound 1 was extended its application for the detection of Cu2+ ion in Vero cells. Compound 1 could be potentially used as an intracellular fluorescent chemosensor for tracking Cu2+ ion.