Enterococcus faecium, Enterococcus faecalis, and Other Enterococcus Species Isolated From Foal Feces in Japan]]>

摘要

Antimicrobial resistant (AMR)Enterococciare an emerging problem for human and animal health. Few studies have focused on the detection of AMR in foals; therefore, we conducted this study to investigate the occurrence of AMREnterococciin foal feces in Japan. A total of 72 fecal samples from healthy foals were collected from seven horse stables, in Hokkaido Prefecture, from June 2015 to January 2016.Enterococciwere isolated and identified using EF Agar Base selective media, and the species confirmed using MALDI Biotyper systems. The minimum inhibitory concentration of nine antimicrobial agents for the isolates was determined based on the Clinical Laboratory Standard Institute guidelines. A total of 183 isolates ofEnterococciwere identified asEnterococcus faecium54.1% (99/183),Enterococcus faecalis16.4% (30/183), and other species 29.5% (54/183). The highest occurrence ofE.?faeciumantibiotic resistant was to erythromycin at 55.6% (55/99), followed by enrofloxacin, kanamycin, and oxytetracycline, at 30.3%, 7.1%, and 4.0%, respectively. In contrast,E.?faecalisisolates showed higher resistant to oxytetracycline 76.7%, kanamycin 46.7%, gentamycin 30.0%, chloramphenicol 16.7%, lincomycin 30.0%, and tylosin 30.0%.E.?faeciumhighly resistant to erythromycin and enrofloxacin but lowly resistant to gentamycin and tylosin, andE.?faecaliswas highly resistant to kanamycin, oxytetracycline, and lincomycin. Antimicrobial resistantE.?faecalis(30.0%) andE.?faecium(4.0%) isolates show multidrug resistant to at least three different classes of antibiotics. Our study indicated that foal feces may act as a source of AMR genes forEnterococcithat may be transmitted to other animals, humans, and the environment.