Shortening the biosensor assay by use of enzyme-containingtemperature-sensitive hydrogels

摘要

To shorten the biosensor assay time, a new technique, based on the increasing of mass transfer rate between the enzyme-containing membrane and the bulk solution as a result of phase transition of stimuli-sensitive matrix (swelling-shrinkage processes) under the action of an outer signal, is proposed. For a model system - glucose oxidase immobilized into thermosensitive poly-N-isopropylacrylamide gel - the total time of the assay procedure can be halved.