Strategic mixed substrate cultivation of microalgae: productivity, respiration, yield, and lipid quality

摘要

Mixotrophic cultivation of three microalgae species, Chlorella sorokiniana, Scenedesmus obliquus and Scenedesmus abundans, was carried out with a mixed substrate containing glucose and acetate (1:1) under indoor and outdoor conditions. For indoor cultivation, the highest biomass productivities for C. sorokiniana, S. obliquus and C. abundans were 331 +/- 11.5, 317 +/- 3.5, and 130.7 +/- 3.1mgL(-1)day(-1), respectively. The key result showed that the highest lipid productivities (29.8-36.2mgL(-1)day(-1)) were obtained for C. sorokiniana for the cases in which acetate was added on day 1 in glucose-mediated cultures. Lipid accumulation was higher (27-36% of dry weight) for S. obliquus and S. abundans. The highest amount of lipid was accumulated for the strategies in which acetate was added on day 1 in glucose-mediated culture. C. sorokiniana showed the highest biomass yields of 0.39gg(-1) as compared to the other two species. For outdoor cultivation, the biomass and lipid productivity values for C. sorokiniana and S. obliquus were 172.8 +/- 4.2 and 183.9 +/- 6.8mgL(-1)day(-1) and 30.5 +/- 2.2 and 26.9 +/- 3.1mgL(-1)day(-1), respectively.