Vancomycin HCl-loaded lauric acid in situ -forming gel with phase inversion for periodontitis treatment

摘要

An antimicrobial agent-loadedin situ-forming gel (ISG) was recently used for periodontitis treatment. This study aimed to use lauric acid, a biocompatible hydrophobic material, as a matrix former. Non-drug and vancomycin HCl-loaded lauric acid-based ISGs were prepared by using various solvents, includingN-methyl pyrrolidone, 2-pyrrolidone and dimethyl sulphoxide (DMSO). Increased concentrations of lauric acid resulted in faster gel self-transformation behaviour. The ISG using DMSO as the solvent showed the fastest transformation with the least water tolerance. Higher concentrations of lauric acid promoted the immediate formation of a dense lauric acid network on the gel surface and obstructed water/solvent diffusion. Through the solvent-exchange mechanism, the hydrophobic property of lauric acid and its rapid surface network formation retarded the diffusion of vancomycin HCl, resulting in prolonged drug release over 6 days. Vancomycin HCl-loaded ISG revealed effective antimicrobial activity against methicillin-resistantStaphylococcus aureusandPorphyromonas gingivalisfor periodontitis treatment. All of the preparations exhibited low viscosity and high injectability, thereby indicating their ease of use. The developed system was found to be effective for periodontal pocket drug delivery for periodontitis treatment.