Development of a fast HPLC-DAD method for simultaneous quantitation of three immunosuppressant drugs in whole blood samples using intelligent chemometrics resolving of coeluting peaks in the presence of blood interferences

摘要

Highlights?A HPLC-DAD method for analysis of immunosuppressants in blood samples is proposed.?Multivariate curve resolution was coupled with a fast and green analytical method.?Proper implementing the constraints was necessary for a satisfactory analysis.?Matrix effect was handled by constructing the matrix-added calibration set.?Quantification was accomplished efficiently using non-sophisticated instrumentation.AbstractThe present study describes a fast high performance liquid chromatography-diode array detection analytical methodology for quantification of tacrolimus, everolimus and cyclosporine A in whole blood samples, with minimum sample preparation steps. A short isocratic chromatographic elution was coupled with second-order calibration using multivariate curve resolution to stablish a sm