Non-targeted metabolomics-guided sildenafil metabolism study in human liver microsomes

摘要

Highlights?We revisited sildenafil metabolism in human liver microsomes using a metabolomics approach.?12 metabolites were identified in HLMs, and elucidated by MS2fragmentation using HR-MS system.?7 Novel metabolites of sildenafil were identified in human liver microsomes.?The global metabolic picture of sildenafil were performed by HR-MS and multivariate data analysis.AbstractMetabolomics combined with high-resolution mass spectrometry (HR-MS) and multivariate data analysis has broad applications in the study of xenobiotic metabolism. Although information about xenobiotic metabolism is essential to understand toxic mechanisms, pharmacokinetic parameters and excretion pathways, it is limited to predict all generated metabolites in biological fluids. Here, we revisited sildenafil metabolism in human liver microsomes using a metabolomics approach to achieve a global picture