Preparative isolation and purification of zearalenone from rice culture by combined use of macroporous resin column and high-speed counter-current chromatography

摘要

Zearalenone is one of the most harmful mycotoxins found in grains and there is a large demand for zearalenone substrate for research purposes.A new separation method was developed for the preparative purification of zearalenone from rice culture of Fusarium graminearum by utilizing macroporous resin column combined with high-speed counter-current chromatography.Zearalenone was adsorpted on XAD-2 resin at 25 °C, neutral pH and a feed flow of 4 BV/h, followed by dynamic desorption by 60% ethanol solution.Further purification was achieved by high-speed counter-current chromatography using an optimized biphasic solvent system.A total of 267 mg of zearalenone crystal was obtained in one single run from 4.2 g of crude extract.The purity of the final product was 98.9% and the total recovery yield of zearalenone in this study was 73.9%.This dual-step purification procedure provided an effective way to obtain the costly mycotoxin for both toxicological and detoxification studies on zearalenone.