THE CHARACTERISTICS OF STAPHYLOCOCCUS AUREUS STRAINS ISOLATED FROM MILK OF MASTITIC COWS, WITH SPECIAL ATTENTION TO THE VIRULENCE FACTORS

摘要

Seventeen Staphylococcus aureus strains isolated from milk of 17 cows with clinical and subclinical mastitis and two Staphylococcus aureus reference strains (PCM 502 and PCM 566 Wood 4) were checked. All investigated strains were tested for their biochemical properties (using API Staph~(TM) by bioMerieux~(R)), production of virulence markers (coagulase, clumping factor, haemolysis, fibrinolysis) and secretion of enterotoxins (using SET-RPLA~(TM) by Oxoid~(R)) The PCR for genetical marker presence - the nuc gene, encoding thermal-stabile nuclease, was used for identification of species S. aureus. All investigated strains revealed typical properties, characteristic for the Staphylococcus aureus, were highly pathogenic and synthesized only enterotoxinD (SED) that proves that the milk of cows with mastitis caused by S. aureus strains might be potential epidemiological risk.