High cell‐density cultivation of phenolic acid decarboxylase‐expressing        <fi xmlns='http://www.wiley.com/namespaces/wiley'>Escherichia coli</fi>Escherichia coli        and 4‐vinylguaiacol bioproduction from ferulic acid by whole‐cell catalysis

Chen Yuheng; Li Lulu; Long Liangkun; Ding Shaojun

摘要

Abstract <section xml:id="jctb5590-sec-0001" numbered="no"> <title type="main">BACKGROUND >4‐vinyl guaiacol (4‐VG) is a high value‐added product widely used in the cosmetic, pharmaceutical, and chemical industries. The practical bioproduction of 4‐VG using phenolic acid decarboxylases has been limited by its relatively high biocatalyst cost and low yield and product concentration. </section> <section xml:id="jctb5590-sec-0002" numbered="no"> <title type="main">RESULTS > In the present study, high‐cell density cultivation was employed to improve the activity and production of phenolic acid decarboxylase from <fi>Bacillus licheniformis</fi> (BLPAD) in recombinant <fi>Escherichia coli</fi> . The factors influencing enzyme production in <fi>E. coli</fi> such as the induction point and temperature for induction and feeding strategies were optimized. The highest BLPAD activity (531 U mL ‐1 ) and productivity (20.4 U mL ‐1 h ‐1 ), respectively, were achieved in a 5 L bioreactor using a glucose exponential feeding strategy with isopropyl β‐D‐thiogalactoside (IPTG) as inducer. Furthermore, a high BLPAD production level (512 U mL ‐1 ) was achieved using lactose as an inducer and continuous lactose feeding. Using a biphasic emulsion system with equal volumes (1 L) of cyclohexane as a organic solvent and a substrate fed‐batch strategy, the concentration and conversion yield of 4‐VG reached 129.9 g L ‐1 (85.6%) in a 5 L bioreactor by whole‐cell biocatalysis, which is the highest reported to date. </section> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“JCTB5590-SEC-0001”编号=“否”> <标题类型=“main”>背景</标题> > 4-乙烯基Guaiacol（4-Vg）是广泛使用的高附加值产品化妆品，制药和化学工业。使用酚酸脱羧酶的4-Vg的实际生物制备已经受其相对高的生物催化剂成本和低产量和产物浓度的限制。</ p> </段> <截面XML：ID =“JCTB5590-SEC-0002”编号为= “否”> <标题类型=“主要”>结果</标题> >在本研究中，使用高细胞密度培养来改善来自<fi>芽孢杆菌的酚醛酸脱羧酶的活性和产生</ Fi>（BLPAD）在重组中<FI>大肠杆菌</ FI>。影响酶生产的因素<fi> e。优化了诸如诱导和喂养策略的诱导点和温度的大肠杆菌进行了优化。最高的BLPAD活性（531u ml -1 -1 -1 -1）和生产率（20.4uml -1 -1 / sup> h h -1 h ）使用葡萄糖指数喂养策略的5L生物反应器，其作为诱导剂的异丙基β-D-硫代酰甲酰胺（IPTG）。此外，使用乳糖作为诱导剂和连续乳糖进料来实现高BLPAD生产水平（512uml -1）。使用具有相同体积（1L）环己烷的双相乳液系统作为有机溶剂和基材FED分批策略，4-Vg的浓度和转化率达到129.9g L -1-1（85.6 ％）通过全细胞生物分析在5L生物反应器中，这是迄今为止最高的。 </ p> </ section> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第8期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen Yuheng&option=202" target="_blank" rel="nofollow">Chen Yuheng;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Lulu&option=202" target="_blank" rel="nofollow">Li Lulu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Long Liangkun&option=202" target="_blank" rel="nofollow">Long Liangkun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ding Shaojun&option=202" target="_blank" rel="nofollow">Ding Shaojun;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>College of Chemical EngineeringNanjing Forestry UniversityNanjing China;</p> <p>College of Chemical EngineeringNanjing Forestry UniversityNanjing China;</p> <p>College of Chemical EngineeringNanjing Forestry UniversityNanjing China;</p> <p>College of Chemical EngineeringNanjing Forestry UniversityNanjing China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=phenolic acid decarboxylase&option=203" rel="nofollow">phenolic acid decarboxylase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=high cell‐density cultivation&option=203" rel="nofollow">high cell‐density cultivation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=whole‐cell catalysis&option=203" rel="nofollow">whole‐cell catalysis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ferulic acid&option=203" rel="nofollow">ferulic acid;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=4‐vinyl guaiacol&option=203" rel="nofollow">4‐vinyl guaiacol;</a> </p> <div class="translation"> 机译：酚酸脱羧酶;高细胞密度栽培;全细胞催化;阿魏酸;4-乙烯基胍醇; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422948.html">High cell‐density cultivation of phenolic acid decarboxylase‐expressing <fi xmlns="http://www.wiley.com/namespaces/wiley">Escherichia coli</fi>Escherichia coli and 4‐vinylguaiacol bioproduction from ferulic acid by whole‐cell catalysis</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen Yuheng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Chen Yuheng,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Lulu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Li Lulu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Long Liangkun&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Long Liangkun,</a> <a href="/journal-foreign-33908/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Chemical Technology & Biotechnology .</a> <span>2018</span><span>,第8期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：高细胞密度培养酚酸脱羧酶的培养<fi xmlns =“http://www.wiley.com/namespaces/wiley”>大肠杆菌</ fi>大肠杆菌和4- vinylguaiacol by-sperlic 细胞催化作用</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422722.html">Production of gamma‐aminobutyric acid by <fc xmlns="http://www.wiley.com/namespaces/wiley"> <fi>Escherichia coli</fi> </fc> <fi xmlns="http://www.wiley.com/namespaces/wiley">Escherichia coli</fi>Escherichia coli using glycerol as a sole carbon source</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hou Chen Yuan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hou Chen Yuan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kang Taek Jin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kang Taek Jin </a> <a href="/journal-foreign-33908/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Chemical Technology & Biotechnology .</a> <span>2018</span><span>,第1期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过<fc xmlns =“http://www.wiley.com/namespaces/wiley”> <fi> escherichia coli </ fi> </ fc> <fi xmlns =“http：// www .wiley.com /命名空间/ wiley“>大肠杆菌</ Fi>大肠杆菌使用甘油作为唯一碳源</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422913.html">Extraction of recombinant proteins from <fc xmlns="http://www.wiley.com/namespaces/wiley"> <fi>Escherichia coli</fi> </fc> <fi xmlns="http://www.wiley.com/namespaces/wiley">Escherichia coli</fi>Escherichia coli by cell disruption with aqueous solutions of surface‐active compounds</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Martins Margarida&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Martins Margarida,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ooi Chien Wei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ooi Chien Wei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Neves Márcia C&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Neves Márcia C,</a> <a href="/journal-foreign-33908/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Chemical Technology & Biotechnology .</a> <span>2018</span><span>,第7期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从<fc xmlns =“http://www.wiley.com/namespaces/wiley”> <fi> escherichia coli </ fi> </ fc> <fi xmlns =“http：//www.wiley .com /命名空间/ wiley“>大肠杆菌”Coli </ Fi>大肠杆菌通过细胞破坏与表面活性化合物的水溶液中断</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-257347_thesis/0705010416417.html">Production of hydroxy-fatty acids and alkanediols by Escherichia coli cells producing fungal cytochrome P450foxy</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kitazume T.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kitazume T.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yamazaki Y.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yamazaki Y.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Idate A. Takaya N.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Idate A. Takaya N. </a> <a href="/conference-foreign-257347/" target="_blank" rel="nofollow" class="tuijian_authcolor">International Conference on Cytochrome P450 .</a> <span>2009</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过大肠杆菌细胞生产羟基 - 脂肪酸和烷二醇，产生真菌细胞色素P450FOXY</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061246355.html">Acidic phospholipid deficiency results in an inhibition of chromosomal replication and cell-cycle specific arrest in Escherichia coli.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fingland, Nicholas K.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Fingland, Nicholas K. </a> <span>2012</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：酸性磷脂缺乏导致大肠杆菌中染色体复制的抑制和细胞周期特异性停滞。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040005182020.html">Bioproduction of High-Concentration 4-Vinylguaiacol Using Whole-Cell Catalysis Harboring an Organic Solvent-Tolerant Phenolic Acid Decarboxylase From Bacillus atrophaeus</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lulu Li&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lulu Li,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liangkun Long&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Liangkun Long,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shaojun Ding&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Shaojun Ding </a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用全细胞催化生物技术生产高浓度的4-乙烯基愈创木酚其中含有来自嗜热芽孢杆菌的耐有机溶剂的酚酸脱羧酶</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000125060002.html">Bioproduction of High-Concentration 4-Vinylguaiacol Using Whole-Cell Catalysis Harboring an Organic Solvent-Tolerant Phenolic Acid Decarboxylase From Bacillus atrophaeus</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lulu Li&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lulu Li,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liangkun Long&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Liangkun Long,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shaojun Ding&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Shaojun Ding </a> <span>2019</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用全细胞催化的高浓度4-乙烯基瓜酚使用来自芽孢杆菌的有机溶剂耐受性酚酸脱羧酶的全细胞催化剂</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201297328673.html">通过重组大肠杆菌的高细胞密度培养热诱导生产人生长激素</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=袁宁&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 袁宁</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=朱春宝&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,朱春宝</a> <span> <a href="/journal-cn-56853/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国医药工业杂志 </a> </span> <span> . 2004</span><span>,第5期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130400503478.html">RECOVERED ANTIBODY (VERSIONS), A METHOD FOR PRODUCING THE ANTIBODY, AN ISOLATED NUCLEIC ACID, AN EXPRESSION CASSETTE (VERSIONS), A PLASMID (VERSIONS), A HOST CELL, A PHARMACEUTICAL PREPARATION, A KIT, A METHOD OF TREATING A PATIENT, AT RISK OF OR SUFFERING FROM ESCHERICHIA COLI INFECTION, AND A DIAGNOSTIC TECHNIQUE FOR DETERMINING ESCHERICHIA COLI INFECTIONS</a> <b>[P]</b> . <span> 外国专利：  RU2724530C2 </span> <span> . 2020-06-23</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：恢复的抗体（版本），一种抗体的生产方法，一种分离的核酸，表达盒（版本），一种质粒（版本），一个宿主细胞，一种药物制剂，一种试剂盒，一种治疗患者的方法大肠埃希氏菌感染的风险或遭受的危险以及确定大肠埃希氏菌感染的诊断技术 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130405829005.html">RECOVERED ANTIBODY (VERSIONS), A METHOD FOR PRODUCING THE ANTIBODY, AN ISOLATED NUCLEIC ACID, AN EXPRESSION CASSETTE (VERSIONS), A PLASMID (VERSIONS), A HOST CELL, A PHARMACEUTICAL PREPARATION, A KIT, A METHOD OF TREATING A PATIENT, AT RISK OF OR SUFFERING FROM ESCHERICHIA COLI INFECTION, AND A DIAGNOSTIC TECHNIQUE FOR DETERMINING ESCHERICHIA COLI INFECTIONS</a> <b>[P]</b> . <span> 外国专利：  RU2016135962A3 </span> <span> . 2018-08-24</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：恢复的抗体（版本），一种抗体的生产方法，一种分离的核酸，一种表达盒（版本），一种质粒（版本），一个宿主细胞，一种药物制剂，一种试剂盒，一种治疗患者的方法大肠埃希氏菌感染的风险或遭受的危险，以及确定大肠埃希氏菌感染的诊断技术 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130436790930.html">Vectors containing guaA gene expressible in Escherichia coli Escherichia cells harboring the same and processes for accumulating 5'-guanylic acid synthetase in a medium using them</a> <b>[P]</b> . <span> 外国专利：  KR100629773B1 </span> <span> . 2006-09-28</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：包含可在大肠杆菌细胞中表达的guaA基因的载体及其载体，以及使用它们在培养基中积累5'-鸟苷酸合成酶的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025422948','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704025422948down" data-source="7," data-out-id="Mw5wNztOFB9PFkF4Oum/tg==," data-f-source-id="7" data-title="High cell‐density cultivation of phenolic acid decarboxylase‐expressing <fi xmlns='http://www.wiley.com/namespaces/wiley'>Escherichia coli</fi>Escherichia coli and 4‐vinylguaiacol bioproduction from ferulic acid by whole‐cell catalysis" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="33908" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="High cell‐density cultivation of phenolic acid decarboxylase‐expressing <fi xmlns='http://www.wiley.com/namespaces/wiley'>Escherichia coli</fi>Escherichia coli and 4‐vinylguaiacol bioproduction from ferulic acid by whole‐cell catalysis"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.8"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.8"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>

High cell‐density cultivation of phenolic acid decarboxylase‐expressing Escherichia coliEscherichia coli and 4‐vinylguaiacol bioproduction from ferulic acid by whole‐cell catalysis

摘要