The <fi xmlns='http://www.wiley.com/namespaces/wiley'>Biomaker</fi>Biomaker : an entry‐level bioprinting device for biotechnological applications

Radtke Carsten Philipp; Hillebrandt Nils; Hubbuch Jürgen

摘要

Abstract <section xml:id="jctb5429-sec-0001" numbered="no"> <title type="main" xml:lang="de">BACKGROUND >3D printing and bioprinting in particular are emerging technologies in the field of biotechnology. The developments of bioprinters and applications lie mostly in the highly observed working fields of tissue engineering and regenerative medicine. Until now only little attention has been paid to the application of 3D bioprinting for the investigation of hydrogel–liquid phase interactions in biotechnological applications. This can mostly be attributed to the need for complex and expensive equipment. </section> <section xml:id="jctb5429-sec-0002" numbered="no"> <title type="main">RESULTS >In this work, an entry‐level bioprinter on the base of a commercially available Fused‐Filament‐Fabrication 3D printer and an easy to handle user interface was designed. This newly developed bioprinter allowed the structuring of bioinks and hydrogels in microwell plates and even complex models were printed. The applicability of the presented printer setup in the field of biotechnology was shown by the encapsulation of β‐galactosidase (EC 3.2.1.23) in poly(ethylene glycol) diacrylate based hydrogels. Subsequently, an automated screening of the biocatalytic conversion of the substrate ONPG by the encapsulated enzyme was executed on a liquid handling station. Under varied pH conditions in the surrounding liquid phase highest substrate turnover rates were detected at pH 3 and pH 5 which is in good accordance with previously reported pH optima of β‐galactosidase. </section> <section xml:id="jctb5429-sec-0003" numbered="no"> <title type="main">CONCLUSION >This approach shows an easy access to 3D bioprinting in the field of biotechnology and t </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“JCTB5429-SEC-0001”编号=“否”> <标题类型=“MAIN”XML：LANG =“de”>背景</标题> > 3D打印和生物印刷，特别是在领域的新兴技术生物技术。生物制剂和应用的发展主要是在组织工程和再生医学的高度观察到的工作领域。到目前为止，只有很少的注意力已经支付给3D BioPlinting用于研究生物技术应用中水凝胶液相相互作用的应用。这主要是归因于对复杂和昂贵的设备的需求。</ p> </ section> <secto XML：ID =“JCTB5429-SEC-0002”编号=“否”> <标题类型=“main”>结果在这项工作中，设计了一种商业上可用的熔丝制造3D打印机基部的入门级Bioplinter和易于处理的用户界面。这种新开发的Bioplinter允许在微孔板中的生物敏和水凝胶的结构，并且打印甚至复杂模型。通过β-半乳糖苷酶（EC 3.2.1.23）中的β-半乳糖苷酶（EC 3.2.1.23）在聚（乙二醇）基水合物水凝胶中的封装显示所呈现的打印机设置的适用性。随后，在液体处理站执行包封酶通过包封酶的生物催化转化的自动筛选。在不同的pH条件下，在pH 3和pH 5中检测到周围的液相最高的基质周转率，所述pH 5和pH 5良好，其与先前报道的β-半乳糖苷酶的pH最佳酶。</ p> </段> <截面XML：ID = “JCTB5429-SEC-0003”编号=“否”> <标题类型=“main”>结论</ title> >本方法显示了在生物技术和T领域的3D Bioplinting轻松访问。 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第3期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Radtke Carsten Philipp&option=202" target="_blank" rel="nofollow">Radtke Carsten Philipp;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hillebrandt Nils&option=202" target="_blank" rel="nofollow">Hillebrandt Nils;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hubbuch Jürgen&option=202" target="_blank" rel="nofollow">Hubbuch Jürgen;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute for Engineering in Life Science Section IV: Biomolecular Separation EngineeringKarlsruhe Institute of TechnologyKarlsruhe Germany;</p> <p>Institute for Engineering in Life Science Section IV: Biomolecular Separation EngineeringKarlsruhe Institute of TechnologyKarlsruhe Germany;</p> <p>Institute for Engineering in Life Science Section IV: Biomolecular Separation EngineeringKarlsruhe Institute of TechnologyKarlsruhe Germany;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=3D bioprinting&option=203" rel="nofollow">3D bioprinting;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=hydrogels&option=203" rel="nofollow">hydrogels;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=encapsulated enzymes&option=203" rel="nofollow">encapsulated enzymes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=high‐throughput screening&option=203" rel="nofollow">high‐throughput screening;</a> </p> <div class="translation"> 机译：3D BioPlinting;水凝胶;包封酶;高通量筛选; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422795.html">The <fi xmlns="http://www.wiley.com/namespaces/wiley">Biomaker</fi>Biomaker : an entry‐level bioprinting device for biotechnological applications</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Radtke Carsten Philipp&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Radtke Carsten Philipp,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hillebrandt Nils&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hillebrandt Nils,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hubbuch Jürgen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hubbuch Jürgen </a> <a href="/journal-foreign-33908/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Chemical Technology & Biotechnology .</a> <span>2018</span><span>,第3期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：<fi xmlns =“http://www.wiley.com/namespaces/wiley”> Biomaker </ Fi> Biomaker：用于生物技术应用的入门级生物印刷设备</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023067228.html">Mixed <fi xmlns="http://www.wiley.com/namespaces/wiley">H</fi>H <sub xmlns="http://www.wiley.com/namespaces/wiley">2</sub>2 / <fi xmlns="http://www.wiley.com/namespaces/wiley">H</fi>H <sub xmlns="http://www.wiley.com/namespaces/wiley"> <fi>∞</fi> </sub> <fi xmlns="http://www.wiley.com/namespaces/wiley">∞</fi>∞ control of synchronization for coupled partial differential systems</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wu Kai‐Ning&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wu Kai‐Ning,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Jian&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wang Jian </a> <a href="/journal-foreign-21736/" target="_blank" rel="nofollow" class="tuijian_authcolor">International Journal of Robust and Nonlinear Control .</a> <span>2017</span><span>,第9期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：混合<fi xmlns =“http://www.wiley.com/namespaces/wiley”> h </ fi> h <sub xmlns =“http://www.wiley.com/namespaces/wiley”> 2 </ sub> 2 / <fi xmlns =“http://www.wiley.com/namespaces/wiley”> h </ fi> h <sub xmlns =“http://www.wiley.com/namespaces/wiley”> <fi>∞</ fi> </ sub> <fi xmlns =“http://www.wiley.com/namespaces/wiley”>∞</ fi>∞耦合偏差系统的同步控制</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422730.html">Enhanced heterologous expression of <fi xmlns="http://www.wiley.com/namespaces/wiley">Trichoderma reesei Cel5A</fi>Trichoderma reesei Cel5A / <fi xmlns="http://www.wiley.com/namespaces/wiley">Cel6A</fi>Cel6A in <fi xmlns="http://www.wiley.com/namespaces/wiley">Pichia pastoris</fi>Pichia pastoris with extracellular co‐expression of <fi xmlns="http://www.wiley.com/namespaces/wiley">Vitreoscilla</fi>Vitreoscilla hemoglobin</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sun Fubao Fuelbiol&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sun Fubao Fuelbiol,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Huimin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yang Huimin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bai Renhui&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Bai Renhui,</a> <a href="/journal-foreign-33908/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Chemical Technology & Biotechnology .</a> <span>2018</span><span>,第1期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：增强的<fi xmlns =“http://www.wiley.com/namespaces/wiley”> trichodermareesei cel5a </ fi> trichoderma reesei cel5a / <fi xmlns =“http://www.wiley.com/ 命名空间/ wiley“> cel6a </ fi> cel6a在<fi xmlns =”http://www.wiley.com/namespaces/wiley“> pichia pastoris </ fi> pichia pastoris与<fi xmlns =的细胞外的共表达式 “http://www.wiley.com/namespaces/wiley"elvitreocilla</fi >vitreoscilla血红蛋白</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-224624_thesis/020516271820.html">Handling smart environment devices, data and services at the semantic level with the FI-WARE core platform</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ramparany Fano&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ramparany Fano,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Galan Marquez Fermin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Galan Marquez Fermin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Soriano Javier&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Soriano Javier,</a> <a href="/conference-foreign-224624/" target="_blank" rel="nofollow" class="tuijian_authcolor">IEEE International Congress on Big Data .</a> <span>2014</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用FI-WARE核心平台在语义级别处理智能环境设备，数据和服务</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100062655614.html">APPLICATIONS OF A FIELD–LEVEL GEOGRAPHIC INFORMATION SYSTEM (FIS) IN PRECISION AGRICULTURE</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=N. Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">N. Zhang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=R. K. Taylor&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">R. K. Taylor </a> <span>2014</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：水平地理信息系统（FIs）在精准农业中的应用</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-lanzhou-medical-college_thesis/0201294908193.html">FI、FIS等载体衍生物对兔血小板聚集和TXB_2释放的影响</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨俊旺&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨俊旺</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=梁永录&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,梁永录</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘茵&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘茵</a> <span> <a href="/journal-cn-50667/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 兰州医学院学报 </a> </span> <span> . 1999</span><span>,第1期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_application-electronic-technique_thesis/0201293616775.html">CDR公司Wi-Fi技术应用于Intempo公司的互联网收音机——Radioscape公司与CSR公司合作，向Intempo公司提供完整的收音机设计</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-8715/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 电子技术应用 </a> </span> <span> . 2008</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_china-food-additives_thesis/020125162916.html">生物酶技术应用于面包焙烤暨生物酶技术应用于溴酸钾的替代方案</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-9438/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国食品添加剂 </a> </span> <span> . 2005</span><span>,第C00期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_spectroscopy-spectral-analysis_thesis/0201271859254.html">FI-KR-FAAS空气混合吸附二次气体分隔洗脱法测定海洋生物样中的痕量铅</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王中瑗&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王中瑗</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张宏康&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张宏康</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=倪志鑫&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,倪志鑫</a> <span> <a href="/journal-cn-11127/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 光谱学与光谱分析 </a> </span> <span> . 2018</span><span>,第011期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_electronic-products_thesis/0201236337721.html">简化开发将催生物联网Wi-Fi应用的大爆发</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王丽英&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王丽英</a> <span> <a href="/journal-cn-8756/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 今日电子 </a> </span> <span> . 2014</span><span>,第008期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-40239_thesis/020222379675.html">蛋白组学技术应用于尿液生物标志物的研究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张庆华&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 张庆华</a> <span> <a href="/conference-cn-40239/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国第一次尿液生物标志物研讨会 </a> <span> <span> . 2015</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020316285616.html">维氏气单胞菌TH0426株△Fis和△Lip的构建及其相关生物学特性分析</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王景霖&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王景霖</a> <span> . 2020</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120113418275.html">用于生物学应用和生物技术应用的优化的受体剪接位点模块</a> <b>[P]</b> . <span> 中国专利： CN113474460A </span> <span> . 2021-10-01</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061202708958.html">用于生物技术应用的非共价型自组织水凝胶基质</a> <b>[P]</b> . <span> 中国专利： CN104736182B </span> <span> . 2018.01.09</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130403245499.html">Wi-Fi blood glucose record analysis system and method connected to server of insurance company by using the blood glucose monitoring device with bluetooth or Wi-Fi and application</a> <b>[P]</b> . <span> 外国专利：  KR20190094571A </span> <span> . 2019-08-14</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用带有蓝牙或wi-fi的血糖监测装置连接至保险公司服务器的wi-fi血糖记录分析系统及方法及应用 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130401242145.html">Wi-Fi antenna for IEEE 802.11ax applications, wireless device, and wireless communication system</a> <b>[P]</b> . <span> 外国专利：  NL2022440B1 </span> <span> . 2020-08-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于IEEE 802.11ax应用，无线设备和无线通信系统的Wi-Fi天线 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130422542733.html">METHOD AND DEVICE FOR PROCESSING WIRELESS FIDELITY (WI-FI) APPLICATIONS OF MOBILE TERMINALS</a> <b>[P]</b> . <span> 外国专利：  WO2012009884A1 </span> <span> . 2012-01-26</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：处理移动终端无线保真（wi-fi）应用的方法和设备 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025422795','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704025422795down" data-source="7," data-out-id="9kBTPWBfVxQO/QvAgyEWOg==," data-f-source-id="7" data-title="The <fi xmlns='http://www.wiley.com/namespaces/wiley'>Biomaker</fi>Biomaker : an entry‐level bioprinting device for biotechnological applications" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="33908" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="The <fi xmlns='http://www.wiley.com/namespaces/wiley'>Biomaker</fi>Biomaker : an entry‐level bioprinting device for biotechnological applications"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.8"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.8"></script> </body> <script> function kefu(customization) { var from = GetQueryString("from"); if (from == null || from == "") { from = "other"; } var url = getWxWorkQrCode(55, from, customization); if (url == "" || typeof url == 'undefined') { url = "https://wework.qpic.cn/wwpic/994838_0LUTS-VqR0quT1N_1675665967/0"; } tipDocGuidance(url, '人工辅助获取外文期刊材料'); } $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>

The BiomakerBiomaker : an entry‐level bioprinting device for biotechnological applications

摘要