Determination of urinary oxalate with alkylamine glass-bound sorghumoxalate oxidase and horseradish peroxidase

摘要

Oxalate in urine was analyzed using sorghum oxalate oxidase and horseradish peroxidase immobilized on alkylamine glass through glutaraldehyde. The minimum detection limit was 0.46 mu g/0.1 ml urine. The recovery of added oxalate was 97.5%. Within and between assay coefficients of variation were < 3.5% and < 6.5% respectively. A good correlation (r=0.9234) was found between oxalate values obtained by a commercial kit method and the present method. The method is unaffected by Cl- and NO3- found in urine.