首页> 外文期刊>Journal of Chemical Technology & Biotechnology >Enhanced permeability of recombinant E. coli E. coliE. coli cells with deep eutectic solvent for transformation of rutin

【24h】

Enhanced permeability of recombinant E. coli E. coliE. coli cells with deep eutectic solvent for transformation of rutin

机译：增强重组的渗透性 e。 COLI e。 Coli e。大肠杆菌具有深层共晶溶剂的细胞用于转化芦丁

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Abstract <section xml:id="jctb5949-sec-0001" numbered="no"> <title type="main" xml:lang="de">BACKGROUND > Whole‐cell catalysis has been widely used because the steps of extracting pure enzyme are omitted and the cost would be greatly saved. However, the efficiency of the whole‐cell catalyst can be limited by the existence of the cell membrane. In the present study, to enhance the binding between an intracellular enzyme and a substrate, ionic liquids (ILs) and deep eutectic solvents (DESs) were used to treat <fc> <fi>Escherichia coli</fi> </fc> BL21‐pET21a‐ rhaB1 cells for isoquercitrin production by the biotransformation of rutin. </section> <section xml:id="jctb5949-sec-0002" numbered="no"> <title type="main">RESULTS > The whole‐cell catalyst exhibited the highest catalytic activity with 6% choline chloride‐urea (ChCl/U) after treatment with different concentrations. The ChCl/U‐treated whole‐cell catalyst was more stable than the crude rhaB1, because its optimum pH was closer to neutral and it demonstrated higher temperature tolerance. Under the best conditions of rutin concentration 0.05?g?L –1 , pH?6.5 and 40?°C, the isoquercitrin yield reached a maximum of 93.05?±?1.3%. Additionally, the whole‐cell catalyst retained 52% enzyme activity after five repeated uses. </section> <section xml:id="jctb5949-sec-0003" numbered="no"> <title type="main">CONCLUSION > These results show that using ChCl/U to increase cell permeability improves the catalytic performance of the whole‐cell catalyst of <fc> <fi>E.?coli</fi> </fc> BL21‐pET21a‐ rhaB1 . This approach has promising potential for the utilization of natural flavonoids. ? 2019 Society of Chemical Industry </section> </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> <第XML：ID =“JCTB5949-SEC-0001”编号=“否”> <title类型=“main”XML：lang =“de”>背景</ title> > 全细胞催化得到广泛使用，因为省略了提取纯酶的步骤，并且成本将大大挽救。然而，全细胞催化剂的效率可以受细胞膜存在的限制。在本研究中，为了增强细胞内酶和基材之间的结合，使用离子液体（ILS）和深共晶溶剂（DESS）治疗 <FC> <fi>大肠杆菌</ fi> </ FC> BL21-PET21A- rhab1 </ i> 通过芦丁的生物转化，对异槲皮蛋白产生的细胞。 </ p> </部分> <第XML：ID =“JCTB5949-SEC-0002”编号=“否”> <标题类型=“main”>结果</ title> > 用不同浓度处理后，全细胞催化剂用6％氯化胆碱 - 脲（CHCl / U）表现出最高的催化活性。 CHCl / U处理的全细胞催化剂比粗rhab1更稳定，因为其最佳pH更接近中性，并且它表现出更高的耐受性。在芦丁浓度的最佳条件下0.05？g？l -1 </ sup> ，pH？6.5和40？°C，异喹硫序产率最高达到93.05°±1.3％。另外，全细胞催化剂保留＆ 52％重复用途后52％的酶活性。 </ p> </部分> <第XML：ID =“JCTB5949-SEC-0003”编号=“否”> <标题类型=“main”>结论</ title> > 这些结果表明，使用CHCl / U增加细胞渗透性，提高了全细胞催化剂的催化性能 <FC> <fi> e.?coli </ fi> </ FC> BL21-PET21A- rhab1 </ i> 。这种方法具有利用天然类黄酮的可能性。还2019年化学工业学会 </ p> </部分> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2020年第2期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Fan&option=202" target="_blank" rel="nofollow">Zhang Fan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhu Chang‐Tong&option=202" target="_blank" rel="nofollow">Zhu Chang‐Tong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Peng Qiang‐Min&option=202" target="_blank" rel="nofollow">Peng Qiang‐Min;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Fang‐Qin&option=202" target="_blank" rel="nofollow">Wang Fang‐Qin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sheng Sheng&option=202" target="_blank" rel="nofollow">Sheng Sheng;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wu Qiong‐Ying&option=202" target="_blank" rel="nofollow">Wu Qiong‐Ying;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Jun&option=202" target="_blank" rel="nofollow">Wang Jun;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>School of BiotechnologyJiangsu University of Science and TechnologyZhenjiang China;</p> <p>School of BiotechnologyJiangsu University of Science and TechnologyZhenjiang China;</p> <p>School of BiotechnologyJiangsu University of Science and TechnologyZhenjiang China;</p> <p>School of BiotechnologyJiangsu University of Science and TechnologyZhenjiang China;</p> <p>School of BiotechnologyJiangsu University of Science and TechnologyZhenjiang China;</p> <p>School of BiotechnologyJiangsu University of Science and TechnologyZhenjiang China;</p> <p>School of BiotechnologyJiangsu University of Science and TechnologyZhenjiang China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biocatalysis&option=203" rel="nofollow">biocatalysis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=isoquercitrin&option=203" rel="nofollow">isoquercitrin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=whole‐cell&option=203" rel="nofollow">whole‐cell;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=permeability&option=203" rel="nofollow">permeability;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ionic liquid&option=203" rel="nofollow">ionic liquid;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=deep eutectic solvent&option=203" rel="nofollow">deep eutectic solvent;</a> </p> <div class="translation"> 机译：生物催化;异喹硫序;全细胞;渗透性;离子液体;深凝胶溶剂; </div> </li> <li> <span class="lefttit">入库时间</span> <span>2022-08-20 08:55:32</span> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422437.html">Enhanced permeability of recombinant <fc > <fi>E. coli</fi> </fc> <fi >E. coli</fi>E. coli cells with deep eutectic solvent for transformation of rutin</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Fan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Fan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhu Chang‐Tong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhu Chang‐Tong,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Peng Qiang‐Min&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Peng Qiang‐Min,</a> <a href="/journal-foreign-33908/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Chemical Technology & Biotechnology .</a> <span>2020</span><span>,第2期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：增强重组的渗透性 <FC> <fi> e。 COLI </ FI> </ FC> <fi> e。 Coli </ Fi> e。大肠杆菌具有深层共晶溶剂的细胞用于转化芦丁</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025556492.html">Bacteriological quality and the prevalence of fi >Salmonella/fi>Salmonella spp. and fi >E. coli/fi>E. coli O157:H7 in ready‐to‐eat foods from Barbados, WI</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hull‐Jackson Carol&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hull‐Jackson Carol,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mota‐Meira Marilaine&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Mota‐Meira Marilaine,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Adesiyun Abiodun&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Adesiyun Abiodun </a> <a href="/journal-foreign-34510/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Food Safety .</a> <span>2019</span><span>,第5期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：细菌学质量和＆＆ salmonella含水的患病率。和＆ fi> e。大肠杆菌＆ / fi> e。 Coli O157：H7在巴巴多斯的即食食品中，Wi</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422913.html">Extraction of recombinant proteins from <fc xmlns="http://www.wiley.com/namespaces/wiley"> <fi>Escherichia coli</fi> </fc> <fi xmlns="http://www.wiley.com/namespaces/wiley">Escherichia coli</fi>Escherichia coli by cell disruption with aqueous solutions of surface‐active compounds</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Martins Margarida&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Martins Margarida,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ooi Chien Wei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ooi Chien Wei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Neves Márcia C&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Neves Márcia C,</a> <a href="/journal-foreign-33908/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Chemical Technology & Biotechnology .</a> <span>2018</span><span>,第7期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从<fc xmlns =“http://www.wiley.com/namespaces/wiley”> <fi> escherichia coli </ fi> </ fc> <fi xmlns =“http：//www.wiley .com /命名空间/ wiley“>大肠杆菌”Coli </ Fi>大肠杆菌通过细胞破坏与表面活性化合物的水溶液中断</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_2014-water-quality-technology-conference-premier_thesis/020511969089.html">A qPCR-based Analysis of Environmental Water Samples for E. coli and toxigenic E. coli</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sharon C. Long&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sharon C. Long,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=India M. Mansour&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">India M. Mansour,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=India M. Mansour&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">India M. Mansour </a> <a href="/conference-foreign-29818/" target="_blank" rel="nofollow" class="tuijian_authcolor">2014 water quality technology conference : The premier conference for water quality professionals around the world .</a> <span>2014</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于qPCR的大肠杆菌和产毒大肠杆菌环境水样品分析</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061269466.html">Examination of E. coli O157 and Salmonella presence and indicator organism levels within an organic farming environment and evaluation of environmental E. coli O157:H7 stress response to starvation and heat.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Adhikari, Achyut.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Adhikari, Achyut. </a> <span>2012</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在有机耕作环境中检查大肠杆菌O157和沙门氏菌的存在以及指示生物水平，并评估环境大肠杆菌O157：H7对饥饿和热的应激反应。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040002128873.html">E. coli Fis Protein Insulates the cbpA Gene from Uncontrolled Transcription</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kiran Chintakayala&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kiran Chintakayala,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shivani S. Singh&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Shivani S. Singh,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Amanda E. Rossiter&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Amanda E. Rossiter,</a> <span>2013</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：大肠杆菌Fis蛋白从不受控制的转录中隔离cbpA基因</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/010006741990.html ">DNA supercoiling is differentially regulated by environmental factors and FIS in Escherichia coli and Salmonella enterica: DNA supercoiling in E. coli and Salmonella</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cameron, Andrew,&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Cameron, Andrew,,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Stoebel, Daniel,&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Stoebel, Daniel,,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dorman, Charles,&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Dorman, Charles, </a> <span>2011</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：DNA超螺旋受环境因素和大肠埃希菌和沙门氏菌中FIS的差异调节：DNA超螺旋在大肠杆菌和沙门氏菌中</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/ntis-science-report_pb_thesis/020711406894.html">Colonization Potentials of Male and Female E. coli K 12 Strains E coli B and Human Fecal E. coli Strains in the Mouse GI Tract</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cohen, P. S. &option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Cohen, P. S. ,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pilsucki, R. W. &option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Pilsucki, R. W. ,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Myhal, M. L. &option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Myhal, M. L. ,</a> <span>1980</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：小鼠胃肠道中雄性和雌性大肠杆菌K12菌株大肠杆菌B和人类粪便大肠杆菌菌株的定殖潜力</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201294239647.html">Creation of Virtual Wi-Fi Access Point and Secured Wi-Fi Pairing, through NFC</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Oussama Stiti&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Oussama Stiti</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Othmen Braham&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Othmen Braham</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guy Pujolle&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Guy Pujolle</a> <span> <a href="/journal-cn-56342/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 通讯、网络与系统学国际期刊（英文） </a> </span> <span> . 2014</span><span>,第6期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_computer-systems-applications_thesis/0201241521305.html">基于K-Means和FCM的增强型Wi-Fi指纹定位策略</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈英&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈英</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=单文杰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,单文杰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨丰玉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨丰玉</a> <span> <a href="/journal-cn-8907/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 计算机系统应用 </a> </span> <span> . 2017</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_catv-technology_thesis/0201238009404.html">基于HFC结构的户外Wi-Fi设计实践</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=温楚欣&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 温楚欣</a> <span> <a href="/journal-cn-8803/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 有线电视技术 </a> </span> <span> . 2017</span><span>,第009期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_electronic-technology-software-engineering_thesis/0201247799655.html">基于NFC技术下的近场通信Wi-Fi传输连接方案</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=焦建军&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 焦建军</a> <span> <a href="/journal-cn-14438/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 电子技术与软件工程 </a> </span> <span> . 2017</span><span>,第018期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_catv-technology_thesis/0201238025678.html">基于HFC有线网络的运营级Wi-Fi无线接入网的性能需求分析及其网络架构</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=田治军&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 田治军</a> <span> <a href="/journal-cn-8803/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 有线电视技术 </a> </span> <span> . 2015</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-13721_thesis/02022967874.html">6×25Fi+FC钢丝绳用钢丝直径比的计算</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曹玉德&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 曹玉德</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=武岳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,武岳</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=董翠兰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,董翠兰</a> <span> <a href="/conference-cn-13721/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 全国金属制品信息网第22届年会 </a> <span> <span> . 2010</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061204321322.html">一种基于广电HFC网络的Wi-Fi实名认证方法</a> <b>[P]</b> . <span> 中国专利： CN108173848B </span> <span> . 2021.04.02</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061202960866.html">使用NFC的WI-FI直连服务方法及其设备</a> <b>[P]</b> . <span> 中国专利： CN104956761B </span> <span> . 2018.08.10</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130432704067.html">METHODS FOR THE IDENTIFICATION OF THE PIG WITH IMMUNITY AGAINST E. COLI F18 INTESTINE COLONIZATION OR INTESTINE DISORDERS RELATED TO E. COLI F18, ISOLATED DNA MOLECULES, A POLYPEPTIDE, A POLYNUCLEOTIDE, MOLECULAR DETERMINATION FOR THE DETECTION OF THE E. COLI F18 RECEPTORS, A KIT FOR THE DETECTION OF THE E. COLI F18 RECEPTORS AND USE OF MUTANT FORM OF THE ALPHA-1,2-FUCOSYL TRANSFERASE 1 GENE OF PIG</a> <b>[P]</b> . <span> 外国专利：  PL195890B1 </span> <span> . 2007-11-30</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：鉴定具有抗E.COLI F18肠道菌落或与E.COLI F18相关的肠道疾病的免疫力的猪的方法，分离的DNA分子，多肽，多核苷酸，分子测定法以检测大肠杆菌。猪大肠杆菌F18受体检测试剂盒和猪ALPHA-1,2-岩藻糖基转移酶1基因突变形式的使用 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130422864887.html">Method of producing a recombinant protein comprising transforming an E. Coli host cell lacking a gene encoding pyrC with a vector comprising a gene encoding the recombinant protein and a gene encoding E. coli pyrC</a> <b>[P]</b> . <span> 外国专利：  NZ567190A </span> <span> . 2012-07-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：产生重组蛋白的方法，包括用包含编码重组蛋白的基因和编码大肠杆菌pyrC的基因的载体转化缺少编码pyrC的基因的大肠杆菌宿主细胞。 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130403036017.html">GENETIC CONSTRUCT BASED ON TWO INDUCIBLE EXPRESSION VECTORS FOR EXPRESSION OF THIAZOLE/OXAZOLE MODIFIED PEPTIDE IN CELLS OF BACTERIA E. COLI; METHOD FOR PRODUCING RECOMBINANT STRAIN OF BACTERIA E. COLI AND METHOD FOR PRODUCING MODIFIED PEPTIDE BASED THEREOF</a> <b>[P]</b> . <span> 外国专利：  RU2694044C2 </span> <span> . 2019-07-08</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于两个诱导表达载体的基因构建用于在大肠杆菌中表达噻唑/恶唑修饰的肽;产大肠杆菌重组菌株的方法及基于其的修饰肽的制备方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025422437','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);">回到顶部</a> </li> <li class="shouye"> <a href="/">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html">关于掌桥</a></li> <li><a href="/help/helpmap.html">资源导航</a></li> <li><a href="/help/helpguide.html">新手指南</a></li> <li><a href="/help/helpcenter.html">常见问题</a></li> <li><a href="/sitemap.html">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704025422437down" data-source="7," data-out-id="inlgJf5GlJTvAqeQqwSxVawAzFoNQCSdgu0Qs1lXx+6zdIY9OIYJh8PtyhzGhlIg," data-f-source-id="7" data-title="Enhanced permeability of recombinant <fc > <fi>E. coli</fi> </fc> <fi >E. coli</fi>E. coli cells with deep eutectic solvent for transformation of rutin" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="33908" id="journalid"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Enhanced permeability of recombinant <fc > <fi>E. coli</fi> </fc> <fi >E. coli</fi>E. coli cells with deep eutectic solvent for transformation of rutin"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.6"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.6"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.6"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.6"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.6"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.6"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.6"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.6"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.6"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.6"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.6"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.6"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); if(sourcetype ==1||sourcetype==4){ getJournal(); } }) </script> </html>