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Optomechanical measurement of the role of lamins in whole cell deformability

机译：光学力学测量层叠在整个细胞变形性中的作用

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Abstract >There is mounting evidence that the nuclear envelope, and particularly the lamina, plays a critical role in the mechanical and regulation properties of the cell and changes to the lamina can have implications for the physical properties of the whole cell. In this study we demonstrate that the optical stretcher can measure changes in the time‐dependent mechanical properties of living cells with different levels of A‐type lamin expression. Results from the optical stretcher shows a decrease in the deformability of cells as the levels of lamin A increases, for cells which grow both adherently and in suspension. Further detail can be probed by combining the optical stretcher with fluorescence microscopy to investigate the nuclear mechanical properties which show a larger decrease in deformability than for the whole cell. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >有核心封套，特别是薄层，在细胞的机械和调节性质中发挥关键作用，并且对薄层的变化可能对整个细胞的物理性质具有影响。在这项研究中，我们证明光学担架可以测量具有不同水平的α层表达的活细胞的时间依赖性机械性能的变化。光学担架的结果显示细胞可变形性的降低，因为粘附性和悬浮液的细胞增加。通过将光学担架与荧光显微镜组合以研究核力学性能来探测进一步的细节，以核肉机械性能，其显示比整个细胞更大的可变形性降低。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33894/'>《Journal of Biophotonics》</a> <b style="margin: 0 2px;">|</b><span>2017年第12期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kolb Thorsten&option=202" target="_blank" rel="nofollow">Kolb Thorsten;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kraxner Julia&option=202" target="_blank" rel="nofollow">Kraxner Julia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Skodzek Kai&option=202" target="_blank" rel="nofollow">Skodzek Kai;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Haug Michael&option=202" target="_blank" rel="nofollow">Haug Michael;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Crawford Dean&option=202" target="_blank" rel="nofollow">Crawford Dean;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Maa? Kendra K.&option=202" target="_blank" rel="nofollow">Maa? Kendra K.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Aifantis Katerina E.&option=202" target="_blank" rel="nofollow">Aifantis Katerina E.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Whyte Graeme&option=202" target="_blank" rel="nofollow">Whyte Graeme;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of PhysicsFriedrich-Alexander-Universit?t Erlangen-NürnbergHenkestrasse 91 91052 Erlangen Germany;</p> <p>Department of PhysicsFriedrich-Alexander-Universit?t Erlangen-NürnbergHenkestrasse 91 91052 Erlangen Germany;</p> <p>Institute of Biological Chemistry Biophysics and BioengineeringHeriot-Watt UniversityEdinburgh EH14 4AS UK;</p> <p>Department of PhysicsFriedrich-Alexander-Universit?t Erlangen-NürnbergHenkestrasse 91 91052 Erlangen Germany;</p> <p>Institute of Biological Chemistry Biophysics and BioengineeringHeriot-Watt UniversityEdinburgh EH14 4AS UK;</p> <p>Division of Molecular GeneticsDeutsches KrebsforschungszentrumIm Neuenheimer Feld 580 69120 Heidelberg Germany;</p> <p>Lab of Mechanics and MaterialsAristotle University of Thessaloniki 54124 Thessaloniki Greece;</p> <p>Department of PhysicsFriedrich-Alexander-Universit?t Erlangen-NürnbergHenkestrasse 91 91052 Erlangen Germany;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/178.html" title="生物物理学">生物物理学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Optical Stretcher&option=203" rel="nofollow">Optical Stretcher;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Single-Cell Analysis&option=203" rel="nofollow">Single-Cell Analysis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cell Mechanics&option=203" rel="nofollow">Cell Mechanics;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nuclear Envelope&option=203" rel="nofollow">Nuclear Envelope;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lamin A&option=203" rel="nofollow">Lamin A;</a> </p> <div class="translation"> 机译：光学担架;单细胞分析;细胞力学;核信封;Lamin a; </div> </li> <li> <span class="lefttit">入库时间</span> <span>2022-08-20 08:55:08</span> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025420063.html">Optomechanical measurement of the role of lamins in whole cell deformability</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kolb Thorsten&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kolb Thorsten,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kraxner Julia&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kraxner Julia,</a> <a 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