CRISPR-Cas9 system: A genome-editing tool with endless possibilities

摘要

The discovery of CRISPR: Cas9 and its application as a powerful gene -editing tool has transformed the world of basic and applied science, especially the molecular biology dome. Also, the smooth, quick, flexible, and very efficient nature of this technology has enabled the biologists to alter the genome of prokaryotes to complex eukaryotic systems, including plants and animals. Using CRISPR and associated tools, investigation, control, and modification of significant biological events have been more accessible than before. These biological scissors are now being used to accelerate breeding programs of crop and livestock, engineer new antimicrobials, and control disease -carrying pathogens. However, like other techniques, these cutters emerged as a double-edged sword and put several challenges to the scientific society. Here in this review article, we summarized the beneficial ap- plication of the CRISPR: Cas9 system and unsafe perception to the society if handled carelessly. We also dis- cussed the limitations and ethical issues related to CRISPR: Cas9 technology.